Preparation of Bioconjugates of Chimeric M13 Phage and Gold Nanorods.

Huan Peng, Irene A Chen

Open access · greenAbstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it

0cells of the map it votes in

2citing papers in PubMed

1.5field-weighted citation impact, top 22% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 citations in OpenAlex.

Systems engineering of engineered live biotherapeutics: A discovery-to-translation framework for streamlining microbiome therapeutic development.Journal of controlled release : official journal of the Controlled Release Society · 2026
Review
Real-TimeACS biomaterials science & engineering · 2025
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 2 countries.

Huan PengCellular Signaling Laboratory, International Research Center for Sensory Biology and Technology of MOST, Key Laboratory of Molecular Biophysics of MOE, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, China. peng_huan@hust.edu.cn.

Irene A ChenDepartment of Chemical and Biomolecular Engineering, Department of Chemistry and Biochemistry, University of California, Los Angeles, CA, USA.

Huazhong University of Science and Technology · CNUniversity of California, Los Angeles · US

Funding

Understanding how bacteriophages affect wound ecologies and developing new tools to harness bacteria-phage interactionsDP2GM123457 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI CHEN, IRENE ANN · 2016 to 2016

$2.3M

Phage-inspired engineering and evolutionR35GM148249 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Irene Ann Chen · 2023 to 2026

$1.5M

NIGMS NIH HHS DP2 GM123457NIGMS NIH HHS R35 GM148249

6 · The paper itself

Abstract

Phage-nanomaterial conjugates are functional bio-nanofibers with various applications. While phage display can select for phages with desired genetically encoded functions and properties, nanomaterials can endow the phages with additional features at nanoscale dimensions. Therefore, combining phages with nanotechnology can construct bioconjugates with unique characteristics. One strategy for filamentous phages is to adsorb nanoparticles onto the side wall, composed of pVIII subunits, through electrostatic interactions. However, a noncovalent approach may cause offloading if the environment changes, potentially causing side effects especially for in vivo applications. Therefore, building stable phage-bioconjugates is an important need. We previously reported the construction of chimeric M13 phage conjugated with gold nanorods, named "phanorods," without weakening the binding affinity to the bacterial host cells. Herein, we give a detailed protocol for preparing the chimeric M13 phage and covalently conjugating gold nanorods to the phage.

Indexed as

InovirusNanotubesBacteriophage M13Cell Surface Display TechniquesGoldGoldBioconjugatesGold nanorodsM13PhagePhage displayPhanorodpIIIpVIII chemical modification

Identifiers

PMID38526728

DOI10.1007/978-1-0716-3798-2_9

PMCPMC11371271

OpenAlexW4393170872

What OpenQuestion holds

Textmetadata

LicenceTDM

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.