Understanding the Structural Requirements of Peptide-Protein Interaction and Applications for Peptidomimetic Development.

Angy Liseth Davalos Macias, Lilian Costa Alecrim, Fabio C L Almeida, Ricardo Jose Giordano

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Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 authors.

Angy Liseth Davalos MaciasBiochemistry Department, Institute of Chemistry, University of Sao Paulo, Sao Paulo, Brazil.

Lilian Costa AlecrimBiochemistry Department, Institute of Chemistry, University of Sao Paulo, Sao Paulo, Brazil.

Fabio C L AlmeidaInstitute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.

Ricardo Jose GiordanoBiochemistry Department, Institute of Chemistry, University of Sao Paulo, Sao Paulo, Brazil. giordano@iq.usp.br.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protein-protein interaction is at the heart of most biological processes, and small peptides that bind to protein binding sites are resourceful tools to explore and understand the structural requirements for these interactions. In that sense, phage display is a well-suited technology to study protein-protein interactions, as it allows for unbiased screening of billions of peptides in search for those that interact with a protein binding domain. Here, we will illustrate how two distinct but complementary approaches, phage display and nuclear magnetic resonance (NMR), can be utilized to unveil structural details of peptide-protein interaction. Finally, knowledge derived from phage mutagenesis and NMR studies can be streamlined for quick peptidomimetic design and synthesis using the retroinversion approach to validate using in vitro and in vivo assays the therapeutic potential of peptides identified by phage display.

Indexed as

PeptidomimeticsCell Surface Display TechniquesPeptide LibraryPeptidesProteinsPeptide LibraryPeptidesPeptidomimeticsProteinsNMRNuclear magnetic resonance (NMR)Nuclear magnetic resonancePeptide and protein interactionPeptidomimetic designPhage displayPhage mutagenesis

Identifiers

PMID38526724

DOI10.3390/ijms22168677

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.