Evidence map›Paper›PMID 38526142›Full record

ArticleMicrobiology spectrum2024

A simple solid media assay for detection of synergy between bacteriophages and antibiotics.

Ethan Khong, Joseph J Oh, Julian M Jimenez, Roland Liu, Sage Dunham, Alisha Monsibais, Alison Rhoads, Pooja Ghatbale, Andrew Garcia, Ana Georgina Cobián Güemes and 10 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
4.6field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Review
  6. Antimicrobial agents and chemotherapy · 2025
    Article
  7. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

20 authors at 2 institutions in 1 country.

Ethan Khong *Department of Pathology, University of California San Diego, La Jolla, California, USA.
Joseph J Oh *Department of Pathology, University of California San Diego, La Jolla, California, USA.ORCID 0009-0005-7377-7827
Julian M JimenezDepartment of Bioengineering, University of California San Diego, La Jolla, California, USA.
Roland LiuDepartment of Pathology, University of California San Diego, La Jolla, California, USA.
Sage DunhamDepartment of Molecular Biology and Biochemistry, University of California Irvine, Irvine, California, USA.ORCID 0000-0002-6338-5038
Alisha MonsibaisDepartment of Molecular Biology and Biochemistry, University of California Irvine, Irvine, California, USA.
Alison RhoadsDepartment of Pathology, University of California San Diego, La Jolla, California, USA.
Pooja GhatbaleDepartment of Pathology, University of California San Diego, La Jolla, California, USA.
Andrew GarciaDepartment of Pathology, University of California San Diego, La Jolla, California, USA.
Ana Georgina Cobián GüemesDepartment of Pathology, University of California San Diego, La Jolla, California, USA.ORCID 0000-0002-0755-5864
Alisha N BlancDepartment of Pathology, University of California San Diego, La Jolla, California, USA.
Megan ChiuDepartment of Pathology, University of California San Diego, La Jolla, California, USA.
Peiting KuoDepartment of Pathology, University of California San Diego, La Jolla, California, USA.
Marissa ProostDepartment of Pathology, University of California San Diego, La Jolla, California, USA.
Ahnika KlineDepartment of Pathology, University of California San Diego, La Jolla, California, USA.
Saima AslamDepartment of Medicine, University of California San Diego, La Jolla, California, USA.ORCID 0000-0002-4051-5629
Robert T SchooleyDepartment of Medicine, University of California San Diego, La Jolla, California, USA.
Katrine WhitesonDepartment of Molecular Biology and Biochemistry, University of California Irvine, Irvine, California, USA.ORCID 0000-0002-5423-6014
Stephanie I FraleyDepartment of Bioengineering, University of California San Diego, La Jolla, California, USA.
David T PrideDepartment of Pathology, University of California San Diego, La Jolla, California, USA.ORCID 0000-0001-8161-6015
University of California San Diego · USUniversity of California, Irvine · US

Funding

Digital High Resolution Melt and Machine Learning for Rapid and Specific Diagnosis in Neonatal SepsisR01AI134982 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI FRALEY, STEPHANIE IRENE · 2018 to 2022
$2.3M
Training in Bioengineering Research and Technology Development in Cardiovascular in Cardiopulmonary Health and DiseaseT32HL160507 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Karen L Christman, Andrew D. McCulloch · 2022 to 2026
$1.6M
NHLBI NIH HHS T32 HL160507NIAID NIH HHS R01 AI134982
6 · The paper itself

Abstract

The emergence of antibiotic-resistant bacteria (ARB) has necessitated the development of alternative therapies to deal with this global threat. Bacteriophages (viruses that target bacteria) that kill ARB are one such alternative. Although phages have been used clinically for decades with inconsistent results, a number of recent advances in phage selection, propagation, and purification have enabled a reevaluation of their utility in contemporary clinical medicine. In most phage therapy cases, phages are administered in combination with antibiotics to ensure that patients receive the standard-of-care treatment. Some phages may work cooperatively with antibiotics to eradicate ARB, as often determined using non-standardized broth assays. We sought to develop a solid media-based assay to assess cooperativity between antibiotics and phages to offer a standardized platform for such testing. We modeled the interactions that occur between antibiotics and phages on solid medium to measure additive, antagonistic, and synergistic interactions. We then tested the method using different bacterial isolates and identified a number of isolates where synergistic interactions were identified. These interactions were not dependent on the specific organism, phage family, or antibiotic used.

Indexed as

Anti-Bacterial AgentsBacteriophagesPhage TherapyBacteriaCulture MediaDrug Resistance, BacterialHumansMicrobial Sensitivity TestsAnti-Bacterial AgentsCulture Mediaantibioticsbacteriophagescooperativitysolid mediasynergy

Identifiers

PMID38526142
PMCPMC11064537
OpenAlexW4393157406

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.