Evidence map›Paper›PMID 38526087›Full record

ArticleJournal of visualized experiments : JoVE2024

Reconstitution of the Bacterial Glutamate Receptor Channel by Encapsulation of a Cell-Free Expression System.

Kyle J Loi, Hossein Moghimianavval, Allen P Liu

Open access · greenAbstract readVideo-Audio Media
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of visualized experiments : JoVE, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Kyle J Loi *Neuroscience Program, University of Michigan, Ann Arbor; Cellular and Molecular Biology Program, University of Michigan, Ann Arbor.
Hossein Moghimianavval *Department of Mechanical Engineering, University of Michigan, Ann Arbor.
Allen P LiuCellular and Molecular Biology Program, University of Michigan, Ann Arbor; Department of Mechanical Engineering, University of Michigan, Ann Arbor; Department of Biomedical Engineering, University of Michigan, Ann Arbor; Department of Biophysics, University of Michigan, Ann Arbor; allenliu@umich.edu.
University of Michigan–Ann Arbor · US

Funding

Development of a mechanosensitive synthetic cell for mediating intercellular communication - Diversity SupplementR01EB030031 · NIBIB · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI LIU, ALLEN PO-CHIH · 2020 to 2024
$1.3M
Mechanosensitive synthetic cell-regulatable hydrogels for tissue engineeringR21AR080363 · NIAMS · UNIVERSITY OF ILLINOIS AT CHICAGO · PI ALSBERG, EBEN, LIU, ALLEN PO-CHIH · 2022 to 2023
$384k
NIAMS NIH HHS R21 AR080363NIBIB NIH HHS R01 EB030031
6 · The paper itself

Abstract

Cell-free expression (CFE) systems are powerful tools in synthetic biology that allow biomimicry of cellular functions like biosensing and energy regeneration in synthetic cells. Reconstruction of a wide range of cellular processes, however, requires successful reconstitution of membrane proteins into the membrane of synthetic cells. While the expression of soluble proteins is usually successful in common CFE systems, the reconstitution of membrane proteins in lipid bilayers of synthetic cells has proven to be challenging. Here, a method for reconstitution of a model membrane protein, bacterial glutamate receptor (GluR0), in giant unilamellar vesicles (GUVs) as model synthetic cells based on encapsulation and incubation of the CFE reaction inside synthetic cells is demonstrated. Utilizing this platform, the effect of substituting the N-terminal signal peptide of GluR0 with proteorhodopsin signal peptide on successful cotranslational translocation of GluR0 into membranes of hybrid GUVs is demonstrated. This method provides a robust procedure that will allow cell-free reconstitution of various membrane proteins in synthetic cells.

Indexed as

Lipid BilayersMembrane ProteinsMembranesProtein Sorting SignalsUnilamellar LiposomesLipid BilayersMembrane ProteinsProtein Sorting SignalsUnilamellar Liposomes

Identifiers

PMID38526087
PMCPMC12289319
OpenAlexW4392755817

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.