ArticleISME communications2024
Blind spots of universal primers and specific FISH probes for functional microbe and community characterization in EBPR systems.
Article in ISME communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Metagenomic characterization of the metabolism, evolution, and global distribution of Candidatus Accumulibacter members in wastewater treatment plants.The ISME journal · 2025Article
- Land conversion to cropland homogenizes variation in soil biota, gene assemblages, and ecological strategies on local and regional scales.The ISME journal · 2025Article
- Novel supplementation of Fe3O4-doped green carbonized nanoparticles on hydrogenases genes and microbial biodiversity for enhancing biohydrogen yield in dark fermentation microbial electrohydrogenesis cells.Journal of industrial microbiology & biotechnology · 2024Article
- Food wastewater treatment using a hybrid biofilm reactor: nutrient removal performance and functional microorganisms on filler biofilm and suspended sludge.RSC advances · 2024Article
- Comparative Analysis of Gut Microbiota between Captive and Wild Long-Tailed Gorals for Ex Situ Conservation.Microorganisms · 2024Article
- Two new clades recovered at high temperatures provide novel phylogenetic and genomic insights intoISME communications · 2024Article
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7 authors.
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Abstract
Fluorescence in situ hybridization (FISH) and 16S rRNA gene amplicon sequencing are commonly used for microbial ecological analyses in biological enhanced phosphorus removal (EBPR) systems, the successful application of which was governed by the oligonucleotides used. We performed a systemic evaluation of commonly used probes/primers for known polyphosphate-accumulating organisms (PAOs) and glycogen-accumulating organisms (GAOs). Most FISH probes showed blind spots and covered nontarget bacterial groups.
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