Evidence map›Paper›PMID 38521591›Full record

ArticleMethods in enzymology2024

Single-molecule observation of G-quadruplex and R-loop formation induced by transcription.

Jihee Hwang, Bradleigh Palmer, Sua Myong

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in enzymology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
6.0field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Jihee HwangProgram in Cellular and Molecular Medicine, Boston Children's Hospital and Harvard Medical School, Boston, MA, United States.
Bradleigh PalmerProgram in Cellular Molecular Developmental Biology and Biophysics, Johns Hopkins University, Baltimore, MD, United States.
Sua MyongProgram in Cellular and Molecular Medicine, Boston Children's Hospital and Harvard Medical School, Boston, MA, United States; Program in Cellular Molecular Developmental Biology and Biophysics, Johns Hopkins University, Baltimore, MD, United States. Electronic address: sua.myong@childrens.harvard.edu.
Boston Children's Hospital · USJohns Hopkins University · US

Funding

Gene Expression Modulated by G4, R-loop and DNA Supercoiling (Supplement for Equipment)R01GM149729 · NIGMS · BOSTON CHILDREN'S HOSPITAL · PI Sua Myong · 2023 to 2026
$1.6M
Quantitative Analysis of G-Quadruplex Folding and its Role in Gene ExpressionR01GM115631 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI MYONG, SUA · 2016 to 2019
$1.2M
NIGMS NIH HHS 1R01GM149729–01NIGMS NIH HHS R01 GM115631NIGMS NIH HHS R01 GM149729
6 · The paper itself

Abstract

Potential G-quadruplex forming sequences (PQS) are enriched in cancer-related genes and immunoglobulin class-switch recombination. They are prevalent in the 5'UTR of transcriptionally active genes, thereby contributing to the regulation of gene expression. We and others previously demonstrated that the PQS located in the non-template strand leads to an R-loop formation followed by a G-quadruplex (G4) formation during transcription. These structural changes increase mRNA production. Here, we present how single-molecule technique was used to observe cotranscriptional G4 and R-loop formation and to examine the impact on transcription, particularly for the initiation and elongation stages.

Indexed as

G-QuadruplexesR-Loop StructuresGene Expression RegulationRNA, MessengerRNA, MessengerAlternative B-DNA structureG4PQS (Potential G-quardfuplex forming sequence)R-loopsingle-molecule FRETsingle-molecule PIFETotal internal refraction fluorescence microscopy (TIRF)Transcription regulation

Identifiers

PMID38521591
PMCPMC11756578
OpenAlexW4390920857

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.