Evidence map›Paper›PMID 38520894›Full record

ArticleVeterinary immunology and immunopathology2024

Quantification of CD3, FoxP3, and granzyme B immunostaining in canine renal cell carcinoma.

Ashleigh Cournoyer, Hayley Amerman, Charles-Antoine Assenmacher, Amy Durham, James A Perry, Allison Gedney, Nicholas Keuler, Matthew J Atherton, Jennifer A Lenz

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary immunology and immunopathology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. The role and mechanism of IL‑35 in myasthenia gravis (Review).International journal of molecular medicine · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Ashleigh CournoyerDepartment of Clinical Studies and Advanced Medicine, University of Pennsylvania, School of Veterinary Medicine, 3900 Spruce Street, Philadelphia, PA 19104, USA.
Hayley AmermanDepartment of Pathobiology, University of Pennsylvania, School of Veterinary Medicine, 3900 Spruce Street, Philadelphia, PA 19104, USA.
Charles-Antoine AssenmacherDepartment of Pathobiology, University of Pennsylvania, School of Veterinary Medicine, 3900 Spruce Street, Philadelphia, PA 19104, USA.
Amy DurhamDepartment of Pathobiology, University of Pennsylvania, School of Veterinary Medicine, 3900 Spruce Street, Philadelphia, PA 19104, USA.
James A PerryDepartment of Clinical Studies and Advanced Medicine, University of Pennsylvania, School of Veterinary Medicine, 3900 Spruce Street, Philadelphia, PA 19104, USA.
Allison GedneyDepartment of Clinical Studies and Advanced Medicine, University of Pennsylvania, School of Veterinary Medicine, 3900 Spruce Street, Philadelphia, PA 19104, USA.
Nicholas KeulerDepartment of Statistics, University of Wisconsin-Madison, 1300 University Ave, Madison, WI 53706, USA.
Matthew J AthertonDepartment of Clinical Studies and Advanced Medicine, University of Pennsylvania, School of Veterinary Medicine, 3900 Spruce Street, Philadelphia, PA 19104, USA; Department of Biomedical Sciences, University of Pennsylvania, School of Veterinary Medicine, 3900 Spruce Street, Philadelphia, PA 19104, USA.
Jennifer A LenzDepartment of Clinical Studies and Advanced Medicine, University of Pennsylvania, School of Veterinary Medicine, 3900 Spruce Street, Philadelphia, PA 19104, USA. Electronic address: jlenz@upenn.edu.
University of Pennsylvania · USUniversity of Wisconsin–Madison · US

Funding

UNIVERSITY OF PENNSYLVANIA CAN CTR SUPPORT GRANTP30CA016520 · NCI · UNIVERSITY OF PENNSYLVANIA · PI Robert H. Vonderheide · 1985 to 2026
$222.3M
Prognostic and Therapeutic Implications of IFNAR1 Signaling on CAR T Cell Therapy for CancerK08CA252619 · NCI · UNIVERSITY OF PENNSYLVANIA · PI ATHERTON, MATTHEW JOHN · 2021 to 2025
$768k
Aperio VERSA Digital Slide Scanner, eSlide Manager Database, and Advanced Image Analysis SoftwareS10OD023465 · OD · UNIVERSITY OF PENNSYLVANIA · PI DURHAM, AMY CLEVELAND · 2018 to 2018
$329k
NCI NIH HHS K08 CA252619NCI NIH HHS P30 CA016520NIH HHS S10 OD023465
6 · The paper itself

Abstract

Tumor-infiltrating lymphocyte (TIL) density plays an important role in anti-tumor immunity and is associated with patient outcome in various human and canine malignancies. As a first assessment of the immune landscape of the tumor microenvironment in canine renal cell carcinoma (RCC), we retrospectively analyzed clinical data and quantified CD3, FoxP3, and granzyme B immunostaining in formalin-fixed paraffin-embedded tumor samples from 16 dogs diagnosed with renal cell carcinoma treated with ureteronephrectomy. Cell density was low for all markers evaluated. Increased numbers of intratumoral FoxP3 labelled (+) cells, as well as decreased granzyme B+: FoxP3+ TIL ratio, were associated with poor patient outcomes. Our initial study of canine RCC reveals that these tumors are immunologically cold and Tregs may play an important role in immune evasion.

Indexed as

Carcinoma, Renal CellCD3 ComplexDog DiseasesForkhead Transcription FactorsGranzymesKidney NeoplasmsLymphocytes, Tumor-InfiltratingAnimalsDogsImmunohistochemistryRetrospective StudiesCD3 ComplexForkhead Transcription FactorsGranzymesCytotoxic T lymphocytesDogGamma-glutamyl transferaseT regulatory cellsTumor-infiltrating lymphocytes

Identifiers

PMID38520894
PMCPMC11056291
OpenAlexW4392882611

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.