Evidence map›Paper›PMID 38520595›Full record

ArticleArchives of virology2024

A multiplex real-time RT-qPCR assay for simultaneous detection of porcine epidemic diarrhea virus, porcine deltacoronavirus, and swine acute diarrhea syndrome coronavirus.

Ji Hye Baek, Yu-Min Lee, Ngoc Duong Vu, Min-Hui Kim, Jincun Zhao, Van Phan Le, Joo Hyuk Cho, Jung-Eun Park

Abstract read
PubMed Publisher
In one paragraph

Article in Archives of virology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
2.4field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 3 countries.

Ji Hye Baek *Molecular Diagnostics Team, Genes Laboratories, 388, Dunchon-daero, Jungwon-gu, Seongnam-si, Gyeonggi-do, 13403, Republic of Korea.
Yu-Min Lee *Molecular Diagnostics Team, Genes Laboratories, 388, Dunchon-daero, Jungwon-gu, Seongnam-si, Gyeonggi-do, 13403, Republic of Korea.
Ngoc Duong Vu *College of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi, Vietnam.
Min-Hui Kim *College of Veterinary Medicine, Chungnam National University, Daejeon, 34134, Republic of Korea.
Jincun ZhaoState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510182, Guangdong, China.
Van Phan LeCollege of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi, Vietnam.
Joo Hyuk ChoMolecular Diagnostics Team, Genes Laboratories, 388, Dunchon-daero, Jungwon-gu, Seongnam-si, Gyeonggi-do, 13403, Republic of Korea.
Jung-Eun ParkCollege of Veterinary Medicine, Chungnam National University, Daejeon, 34134, Republic of Korea. jepark@cnu.ac.kr.ORCID http://orcid.org/0000-0002-0423-6147
Chungnam National University · KRVietnam National University of Agriculture · VNFirst Affiliated Hospital of Guangzhou Medical University · CN

Funding

Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry 122012-2Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry 122060-2
6 · The paper itself

Abstract

Porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome coronavirus (SADS-CoV) cause intestinal diseases with similar manifestations in suckling piglets. In this study, we developed a multiplex real-time PCR for differential diagnosis of PEDV, PDCoV, and SADS-CoV. The assay demonstrated high specificity with a detection limit of 5 copies/µl for each virus. The assay specifically detected PEDV, PDCoV, and SADS-CoV and excluded all other swine pathogens circulating in pigs. Furthermore, the assay exhibited satisfactory performance in analyzing clinical samples. The data indicate that the newly developed multiplex real-time PCR method can be applied for differential diagnosis of porcine enteric coronaviruses.

Indexed as

AlphacoronavirusCoronavirus InfectionsDeltacoronavirusPorcine epidemic diarrhea virusSwine DiseasesAnimalsDiarrheaSensitivity and SpecificitySwine

Identifiers

PMID38520595
OpenAlexW4393113787

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.