Evidence map›Paper›PMID 38519993›Full record

ArticleParasites & vectors2024

The crosstalk between cholangiocytes and hepatic stellate cells promotes the progression of epithelial-mesenchymal transition and periductal fibrosis during Clonorchis sinensis infection.

Junyeong Yi, Ji Hoon Jeong, Jihee Won, Seok Chung, Jhang Ho Pak

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Parasites & vectors, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.9field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Food and waterborne parasitology · 2026
    Article
  3. Editorial: Parasite-induced liver diseases, volume II.Frontiers in cellular and infection microbiology · 2026
    Article
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Junyeong YiDepartment of Biochemistry, Asan Medical Institute of Convergence Science and Technology (AMIST), University of Ulsan College of Medicine and Asan Medical Center (AMC), 88 Olympic-Ro 43-Gil, Songpa-gu, Seoul, 05505, Republic of Korea.
Ji Hoon JeongDepartment of Biochemistry, Asan Medical Institute of Convergence Science and Technology (AMIST), University of Ulsan College of Medicine and Asan Medical Center (AMC), 88 Olympic-Ro 43-Gil, Songpa-gu, Seoul, 05505, Republic of Korea.
Jihee WonSchool of Mechanical Engineering, Korea University, 145 Anam-Ro, Seongbuk-gu, Seoul, 02841, Republic of Korea.
Seok ChungSchool of Mechanical Engineering, Korea University, 145 Anam-Ro, Seongbuk-gu, Seoul, 02841, Republic of Korea.
Jhang Ho PakDepartment of Biochemistry, Asan Medical Institute of Convergence Science and Technology (AMIST), University of Ulsan College of Medicine and Asan Medical Center (AMC), 88 Olympic-Ro 43-Gil, Songpa-gu, Seoul, 05505, Republic of Korea. jhpak@amc.seoul.kr.
Ulsan College · KRKorea University · KR

Funding

National Research Foundation of Korea 2020R1F1A1071492
6 · The paper itself

Abstract

ABSTRACT:

backgroundClonorchis sinensis infection is one of the risk factors that provokes chronic inflammation, epithelial hyperplasia, periductal fibrosis and even cholangiocarcinoma (CCA). Disrupted or aberrant intercellular communication among liver-constituting cells leads to pathological states that cause various hepatic diseases. This study was designed to investigate the pathological changes caused by C. sinensis excretory-secretory products (ESPs) in non-cancerous human cell lines (cholangiocytes [H69 cell line] and human hepatic stellate cells [LX2 cell line]) and their intercellular crosstalk, as well the pathological changes in infected mouse liver tissues.

methodsThe cells were treated with ESPs, following which transforming growth factor beta 1 (TGF-β1) and interleukin-6 (IL-6) secretion levels and epithelial-mesenchymal transition (EMT)- and fibrosis-related protein expression were measured. The ESP-mediated cellular motility (migration/invasion) between two cells was assessed using the Transwell and three-dimensional microfluidic assay models. The livers of C. sinensis-infected mice were stained using EMT and fibrotic marker proteins.

resultsTreatment of cells with ESPs increased TGF-β1 and IL-6 secretion and the expression of EMT- and fibrosis-related proteins. The ESP-mediated mutual cell interaction further affected the cytokine secretion and protein expression levels and promoted cellular motility. N-cadherin overexpression and collagen fiber deposition were observed in the livers of C. sinensis-infected mice.

conclusionsThese findings suggest that EMT and biliary fibrosis occur through intercellular communication between cholangiocytes and hepatic stellate cells during C. sinensis infection, promoting malignant transformation and advanced hepatobiliary abnormalities.

Indexed as

Bile Duct NeoplasmsClonorchiasisClonorchis sinensisAnimalsBile Ducts, IntrahepaticEpithelial-Mesenchymal TransitionFibrosisHepatic Stellate CellsHumansInterleukin-6MiceTransforming Growth Factor beta1Interleukin-6Transforming Growth Factor beta1CholangiocytesClonorchis sinensisEpithelial-mesenchymal transitionExcretory-secretory productsHepatic stellate cellsIntercellular crosstalkPeriductal fibrosis

Identifiers

PMID38519993
PMCPMC10958959
OpenAlexW4393095600

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.