Evidence map›Paper›PMID 38518781›Full record

ArticleStem cell reports2024

Direct programming of human pluripotent stem cells into endothelial progenitors with SOX17 and FGF2.

Michael W Ream, Lauren N Randolph, Yuqian Jiang, Yun Chang, Xiaoping Bao, Xiaojun Lance Lian

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.2field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Michael W ReamDepartment of Biomedical Engineering, Pennsylvania State University, University Park, PA 16802, USA.
Lauren N RandolphDepartment of Biomedical Engineering, Pennsylvania State University, University Park, PA 16802, USA.
Yuqian JiangDepartment of Biomedical Engineering, Pennsylvania State University, University Park, PA 16802, USA.
Yun ChangDavidson School of Chemical Engineering, Purdue University, West Lafayette, IN 47907, USA.
Xiaoping BaoDavidson School of Chemical Engineering, Purdue University, West Lafayette, IN 47907, USA. Electronic address: bao61@purdue.edu.
Xiaojun Lance LianDepartment of Biomedical Engineering, Pennsylvania State University, University Park, PA 16802, USA; Department of Biology, Pennsylvania State University, University Park, PA 16802, USA; The Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA 16802, USA. Electronic address: lian@psu.edu.
Pennsylvania State University · USPurdue University West Lafayette · US

Funding

Engineer Biomimetic Microfluidic Models to Investigate and Reprogram Tumor Associated Neutrophils for Cancer TherapyR37CA265926 · NCI · PURDUE UNIVERSITY · PI Xiaoping Bao · 2022 to 2026
$1.7M
Stem cell immunoengineering for universal cardiac therapy via CRISPR-Cas9R21EB026035 · NIBIB · PENNSYLVANIA STATE UNIVERSITY, THE · PI LIAN, XIAOJUN · 2018 to 2020
$567k
ModRNA-based Direct Programming of Universal Donor hiPSCs into Immune Evasive Beta CellsR56DK133147 · NIDDK · PENNSYLVANIA STATE UNIVERSITY, THE · PI LIAN, XIAOJUN · 2023 to 2024
$465k
NCI NIH HHS R37 CA265926NIBIB NIH HHS R21 EB026035NIDDK NIH HHS R56 DK133147
6 · The paper itself

Abstract

Transcription factors (TFs) are pivotal in guiding stem cell behavior, including their maintenance and differentiation. Using single-cell RNA sequencing, we investigated TFs expressed in endothelial progenitors (EPs) derived from human pluripotent stem cells (hPSCs) and identified upregulated expression of SOXF factors SOX7, SOX17, and SOX18 in the EP population. To test whether overexpression of these factors increases differentiation efficiency, we established inducible hPSC lines for each SOXF factor and found only SOX17 overexpression robustly increased the percentage of cells expressing CD34 and vascular endothelial cadherin (VEC). Conversely, SOX17 knockdown via CRISPR-Cas13d significantly compromised EP differentiation. Intriguingly, we discovered SOX17 overexpression alone was sufficient to generate CD34

Indexed as

Endothelial CellsFibroblast Growth Factor 2Pluripotent Stem CellsSOXF Transcription FactorsAntigens, CD34Cell DifferentiationHumansAntigens, CD34Fibroblast Growth Factor 2SOX17 protein, humanSOX18 protein, humanSOX7 protein, humanSOXF Transcription Factors

Identifiers

PMID38518781
PMCPMC11096437
OpenAlexW4393038176

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.