Evidence map›Paper›PMID 38517574›Full record

ArticleAntonie van Leeuwenhoek2024

Targeting methanotrophs and isolation of a novel psychrophilic Methylobacter species from a terrestrial Arctic alkaline methane seep in Lagoon Pingo, Central Spitsbergen (78° N).

Shalaka K Patil, Tajul Islam, Alexander Tveit, Andrew Hodson, Lise Øvreås

Open access · hybridAbstract read
In one paragraph

Article in Antonie van Leeuwenhoek, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.0field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Review
  5. International journal of systematic and evolutionary microbiology · 2025
    Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Shalaka K PatilDepartment of Biological Sciences, University of Bergen, Postboks 7803, 5020, Bergen, Norway. shalaka.patil@uib.no.ORCID http://orcid.org/0000-0002-1341-4208
Tajul IslamDepartment of Biological Sciences, University of Bergen, Postboks 7803, 5020, Bergen, Norway.
Alexander TveitDepartment of Arctic and Marine Biology, The Arctic University of Tromsø, 9037, Tromsø, Norway.
Andrew HodsonUniversity Centre in Svalbard, 9171, Longyearbyen, Norway.
Lise ØvreåsDepartment of Biological Sciences, University of Bergen, Postboks 7803, 5020, Bergen, Norway.
University of Bergen · NOBjerknes Centre for Climate Research · NOUiT The Arctic University of Norway · NO

Funding

Norges Forskningsråd 294764
6 · The paper itself

Abstract

The microbial diversity associated with terrestrial groundwater seepage through permafrost soils is tightly coupled to the geochemistry of these fluids. Terrestrial alkaline methane seeps from Lagoon Pingo, Central Spitsbergen (78°N) in Norway, with methane-saturated and oxygen-limited groundwater discharge providing a potential habitat for methanotrophy. Here, we report on the microbial community's comparative analyses and distribution patterns at two sites close to Lagoon Pingo's methane emission source. To target methane-oxidizing bacteria from this system, we analysed the microbial community pattern of replicate samples from two sections near the main methane seepage source. DNA extraction, metabarcoding and subsequent sequencing of 16S rRNA genes revealed microbial communities where the major prokaryotic phyla were Pseudomonadota (42-47%), Gemmatimonadota (4-14%) and Actinobacteriota (7-11%). Among the Pseudomonadota, members of the genus Methylobacter were present at relative abundances between 1.6 and 4.7%. Enrichment targeting the methane oxidising bacteria was set up using methane seep sediments as inoculum and methane as the sole carbon and energy source, and this resulted in the isolation of a novel psychrophilic methane oxidizer, LS7-T4A

Indexed as

MethaneMethylococcaceaeDNA, BacterialFatty AcidsPhylogenyRNA, Ribosomal, 16SSequence Analysis, DNASvalbardDNA, BacterialFatty AcidsMethaneRNA, Ribosomal, 16SCold methane seepsMethanotrophsMethylobacterPingospMMOSvalbard

Identifiers

PMID38517574
PMCPMC10959801
OpenAlexW4393095484

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.