Evidence map›Paper›PMID 38516638›Full record

ArticleBioelectricity2024

Optical Estimation of Membrane Potential Values Using Fluorescence Lifetime Imaging Microscopy and Hybrid Chemical-Genetic Voltage Indicators.

Anneliese M M Gest, Vincent Grenier, Evan W Miller

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioelectricity, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Anneliese M M GestDepartment of Chemistry, University of California, Berkeley, California, USA.ORCID https://orcid.org/0000-0002-0923-2989
Vincent GrenierDepartment of Chemistry, University of California, Berkeley, California, USA.
Evan W MillerDepartment of Chemistry, University of California, Berkeley, California, USA.ORCID https://orcid.org/0000-0002-6556-7679

Funding

Interrogating Neuronal Membrane Potential Dynamics with Optical Voltage SensorsR01NS098088 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Evan Walker Miller · 2017 to 2026
$4.4M
NINDS NIH HHS R01 NS098088
6 · The paper itself

Abstract

Introduction: Membrane potential ( Objectives: To address this problem, we applied a chemical-genetic voltage imaging approach to FLIM to enable optical estimation of membrane potential values from genetically defined cells. Results: In this report, we detail the characterization and evaluation of two of these systems in mammalian cells. We further validate the use of a FLIM-based chemical genetic voltage indicator in mammalian neurons. Conclusions: Finally, we discuss opportunities for future improvements to chemical-genetic FLIM-based voltage indicators.

Indexed as

FLIMfluorescencevoltage sensitive fluorophores

Identifiers

PMID38516638
PMCPMC10951690

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.