ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2024
Alternative Splicing Underpins the ALMT9 Transporter Function for Vacuolar Malic Acid Accumulation in Apple.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 21 citations in OpenAlex.
- Alternative splicing rewires banana aroma biosynthesis.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Sex chromosome evolution mediated by a large inversion and a possible switch of the sex determination gene.Genome biology · 2026Article
- Banana aroma is a result of acetohydroxyacid synthase and isopropylmalate synthase alternative isoforms that bypass feedback inhibition.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Molecular orchestration of malate and citrate metabolism: regulatory networks governing organic acid dynamics and fruit quality attributes.Horticulture research · 2026Article
- Overexpression of a Tonoplast Malate Transporter Gene Leads to Enhanced Anthocyanin Biosynthesis in Apple.Plant biotechnology journal · 2026Article
- Comprehensive analysis of alternative splicing and transcriptome diversity in apple using long-read sequencing.Frontiers in plant science · 2026Article
- Malate metabolism in horticultural crops: mechanistic insights and agricultural practices for quality improvement.Molecular horticulture · 2025Review
- Aluminum-activated malate transporter family member CsALMT6 mediates fluoride resistance in tea plants (Horticulture research · 2025Article
- Genome-Wide Identification, Molecular Evolution, and Expression Divergence ofFood science & nutrition · 2025Article
- CBL1/CIPK23 phosphorylates tonoplast sugar transporter TST2 to enhance sugar accumulation in sweet orange (Citrus sinensis).Journal of integrative plant biology · 2025Article
- Article
- A linker histone acts as a transcription factor to orchestrate malic acid accumulation in apple in response to sorbitol.The Plant cell · 2024Article
- Sorbitol signaling: Linker histone MdH1.1 modulates malic acid buildup in apple.The Plant cell · 2024Article
- Multi-Omics Analysis Uncovers the Mechanism for Enhanced Organic Acid Accumulation in Peach (Plants (Basel, Switzerland) · 2024Article
- The art of tartness: the genetics of organic acid content in fresh fruits.Horticulture research · 2024Article
- Alternative Splicing Underpins the ALMT9 Transporter Function for Vacuolar Malic Acid Accumulation in Apple.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Abscisic Acid Lowers Postharvest Grape Acidity via PP2C-ALMT9-Dependent Malate Transport.Physiologia plantarumArticle
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
Abstract
Vacuolar malic acid accumulation largely determines fruit acidity, a key trait for the taste and flavor of apple and other fleshy fruits. Aluminum-activated malate transporter 9 (ALMT9/Ma1) underlies a major genetic locus, Ma, for fruit acidity in apple, but how the protein transports malate across the tonoplast is unclear. Here, it is shown that overexpression of the coding sequence of Ma1 (Ma1α) drastically decreases fruit acidity in "Royal Gala" apple, leading to uncovering alternative splicing underpins Ma1's function. Alternative splicing generates two isoforms: Ma1β is 68 amino acids shorter with much lower expression than the full-length protein Ma1α. Ma1β does not transport malate itself but interacts with the functional Ma1α to form heterodimers, creating synergy with Ma1α for malate transport in a threshold manner (When Ma1β/Ma1α ≥ 1/8). Overexpression of Ma1α triggers feedback inhibition on the native Ma1 expression via transcription factor MYB73, decreasing the Ma1β level well below the threshold that leads to significant reductions in Ma1 function and malic acid accumulation in fruit. Overexpression of Ma1α and Ma1β or genomic Ma1 increases both isoforms proportionally and enhances fruit malic acid accumulation. These findings reveal an essential role of alternative splicing in ALMT9-mediated malate transport underlying apple fruit acidity.
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Registered trials
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