Evidence map›Paper›PMID 38514830›Full record

ArticleScientific reports2024

Involvement of TGFBI-TAGLN axis in cancer stem cell property of head and neck squamous cell carcinoma.

Motoharu Sarubo, Yasuhiro Mouri, Akira Moromizato, Azusa Yamada, Shengjan Jin, Wenhua Shao, Hiroko Hagita, Keiko Miyoshi, Yasusei Kudo

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.5field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Transcriptome Analysis and Histological Validation Identified the HO-1-u-1 Cell Line as a Model for Perineural Invasion in Oral Squamous Cell Carcinoma.Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Motoharu SaruboDepartment of Oral Bioscience, Tokushima University Graduate School of Biomedical Sciences, Tokushima, Japan.
Yasuhiro MouriDepartment of Oral Bioscience, Tokushima University Graduate School of Biomedical Sciences, Tokushima, Japan.
Akira MoromizatoDepartment of Oral Bioscience, Tokushima University Graduate School of Biomedical Sciences, Tokushima, Japan.
Azusa YamadaDepartment of Oral Bioscience, Tokushima University Graduate School of Biomedical Sciences, Tokushima, Japan.
Shengjan JinDepartment of Oral Bioscience, Tokushima University Graduate School of Biomedical Sciences, Tokushima, Japan.
Wenhua ShaoDepartment of Oral Bioscience, Tokushima University Graduate School of Biomedical Sciences, Tokushima, Japan.
Hiroko HagitaDepartment of Oral Bioscience, Tokushima University Graduate School of Biomedical Sciences, Tokushima, Japan.
Keiko MiyoshiDepartment of Oral Bioscience, Tokushima University Graduate School of Biomedical Sciences, Tokushima, Japan.
Yasusei KudoDepartment of Oral Bioscience, Tokushima University Graduate School of Biomedical Sciences, Tokushima, Japan. yasusei@tokushima-u.ac.jp.
Tokushima University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Head and neck squamous cell carcinoma (HNSCC) is a significant healthcare burden globally. Previous research using single-cell transcriptome analysis identified TGFBI as a crucial marker for the partial-epithelial-mesenchymal transition (partial-EMT) program. However, the precise role of TGFBI in HNSCC progression remains unclear. Therefore, our study aimed to clarify the impact of TGFBI on the malignant behavior of HNSCC cells. Through RNA-sequencing data from the TCGA database, we validated that increased TGFBI expression correlates with a higher occurrence of lymph node metastasis and unfavorable prognosis in HNSCC cases. Functional experiments demonstrated that TGFBI overexpression enhances the ability of sphere formation, indicating stem-cell-like properties. Conversely, TGFBI depletion reduces sphere formation and suppresses the expression of cancer stem cell (CSC) markers. RNA-sequencing analysis of TGFBI-overexpressing and control HNSCC cells revealed TAGLN as a downstream effector mediating TGFBI-induced sphere formation. Remarkably, TAGLN depletion abolished TGFBI-induced sphere formation, while its overexpression rescued the suppressed sphere formation caused by TGFBI depletion. Moreover, elevated TAGLN expression showed correlations with the expression of TGFBI and partial-EMT-related genes in HNSCC cases. In conclusion, our findings suggest that TGFBI may promote CSC properties through the upregulation of TAGLN. These novel insights shed light on the involvement of the TGFBI-TAGLN axis in HNSCC progression and hold implications for the development of targeted therapies.

Indexed as

Carcinoma, Squamous CellHead and Neck NeoplasmsCell Line, TumorEpithelial-Mesenchymal TransitionGene Expression Regulation, NeoplasticHumansNeoplastic Stem CellsRNASquamous Cell Carcinoma of Head and NeckRNACancer stem cellHead and neck squamous cell carcinomaPartial-EMTTGFBITransgelin

Identifiers

PMID38514830
PMCPMC10957997
OpenAlexW4393046337

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.