Optimized Calcium-Phosphate-Based Co-transfection of Tau and tdTomato into Human iPSC-Derived Neurons for the Study of Intracellular Distribution of Wild-type and Mutant Human Tau.

Panagiotis Lilis, Mohamed Aghyad Al Kabbani, Hans Zempel

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

1.5field-weighted citation impact, top 25% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Panagiotis LilisInstitute for Human Genetics & Center for Molecular Medicine Cologne, University Hospital of Cologne, Faculty of Medicine, University of Cologne, Cologne, Germany.

Mohamed Aghyad Al KabbaniInstitute for Human Genetics & Center for Molecular Medicine Cologne, University Hospital of Cologne, Faculty of Medicine, University of Cologne, Cologne, Germany.

Hans ZempelInstitute for Human Genetics & Center for Molecular Medicine Cologne, University Hospital of Cologne, Faculty of Medicine, University of Cologne, Cologne, Germany. hans.zempel@uk-koeln.de.

University Hospital Cologne · DEUniversity of Cologne · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The study of Tau protein in disease-relevant neuronal cells in culture requires efficient delivery systems for transfection of exogenous Tau and also modulators and interactors of Tau. Transfection of cultivated cells using calcium phosphate precipitation is a simple and cost-effective approach, also for difficult-to-transfect and sensitive cells such as primary neurons. Because of its low cell toxicity and ease of use, the Ca

Indexed as

Induced Pluripotent Stem CellsRed Fluorescent Proteintau ProteinsCalciumCalcium PhosphatesHumansNeuronsPhosphatesTransfectionCalciumcalcium phosphateCalcium PhosphatesPhosphatesRed Fluorescent Proteintau ProteinstdTomatoCalcium-phosphate transfectionCo-transfectioniPSC-derived neuronsTautdTomatoTransfection efficiency

Identifiers

PMID38512689

DOI10.1101/pdb.top096255

OpenAlexW4393051316

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.