Intranasal Nose-to-Brain Drug Delivery via the Olfactory Region in Mice: Two In-Depth Protocols for Region-Specific Intranasal Application of Antibodies and for Expression Analysis of Fc Receptors via In Situ Hybridization in the Nasal Mucosa.

Helena Herzog, Sara Glöckler, Johannes Flamm, Simone Ladel, Frank Maigler, Claudia Pitzer, Katharina Schindowski

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it

0cells of the map it votes in

3citing papers in PubMed

6.0field-weighted citation impact, top 4% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

Intranasal Drug Delivery Technology in the Treatment of Central Nervous System Diseases: Challenges, Advances, and Future Research Directions.Pharmaceutics · 2025
Review
Development and Characterization of a Primary Ciliated Porcine Airway Model for the Evaluation of In Vitro Mucociliary Clearance and Mucosal Drug Delivery.Pharmaceutics · 2025
Article
Characterization of a primary cellular airway model for inhalative drug delivery in comparison with the established permanent cell lines CaLu3 and RPMI 2650.In vitro models · 2024
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Helena HerzogInstitute of Applied Biotechnology, University of Applied Science Biberach, Biberach, Germany.

Sara GlöcklerInstitute of Applied Biotechnology, University of Applied Science Biberach, Biberach, Germany.

Johannes FlammInstitute of Applied Biotechnology, University of Applied Science Biberach, Biberach, Germany.

Simone LadelInstitute of Applied Biotechnology, University of Applied Science Biberach, Biberach, Germany.

Frank MaiglerInstitute of Applied Biotechnology, University of Applied Science Biberach, Biberach, Germany.

Claudia PitzerInterdisciplinary Neurobehavioral Core, Heidelberg University, Heidelberg, Germany.

Katharina SchindowskiInstitute of Applied Biotechnology, University of Applied Science Biberach, Biberach, Germany. schindowski@hochschule-bc.de.

University of Applied Sciences Biberach · DEUniversität Ulm · DEHeidelberg University · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A region-specific catheter-based intranasal administration method was successfully developed, established, and validated as reported previously. By using this method, drugs can be applicated specifically to the olfactory region. Thereby, intranasally administered drugs could be delivered via neuronal connections to the central nervous system. Here, we present a detailed protocol with a step-by-step procedure for nose-to-brain delivery via the olfactory mucosa.Fc receptors such as the neonatal Fc receptor (FcRn) and potentially Fcγ receptor IIb (FcγRIIb) are involved in the uptake and transport of antibodies via the olfactory nasal mucosa. To better characterize their expression levels and their role in CNS drug delivery via the nose, an in situ hybridization (ISH) protocol was adapted for nasal mucosa samples and described in abundant details.

Indexed as

BrainNasal MucosaAdministration, IntranasalAnimalsAntibodiesDrug Delivery SystemsIn Situ HybridizationMicePharmaceutical PreparationsReceptors, FcAntibodiesPharmaceutical PreparationsReceptors, FcCNS deliveryIn situ hybridizationIntranasal deliveryNose-to-brain drug deliveryOlfactory mucosa

Identifiers

PMID38512678

DOI10.1093/ajcp/96.4.470

OpenAlexW4393051809

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.