Evidence map›Paper›PMID 38511503›Full record

ArticleProtein science : a publication of the Protein Society2024

Efficient and economic protein labeling for NMR in mammalian expression systems: Application to a preT-cell and T-cell receptor protein.

Robert J Mallis, Jonathan J Lee, Arjen Van den Berg, Kristine N Brazin, Thibault Viennet, Jonathan Zmuda, Melissa Cross, Denitsa Radeva, Ricard Rodriguez-Mias, Judit Villén and 3 more

Open access · greenAbstract read
In one paragraph

Article in Protein science : a publication of the Protein Society, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
5.2field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Atomistic TCR-ligand interactions instruct memory T-cell differentiation.bioRxiv : the preprint server for biology · 2025
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 2 countries.

Robert J MallisLaboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA.ORCID 0000-0002-2087-9468
Jonathan J LeeLaboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
Arjen Van den BergThermo Fisher Scientific, Frederick, Maryland, USA.
Kristine N BrazinLaboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
Thibault ViennetDepartment of Cancer Biology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA.ORCID 0000-0001-5349-0179
Jonathan ZmudaThermo Fisher Scientific, Frederick, Maryland, USA.
Melissa CrossThermo Fisher Scientific, Frederick, Maryland, USA.
Denitsa RadevaFaculty of Chemistry and Pharmacy, Sofia University, Sofia, Bulgaria.
Ricard Rodriguez-MiasDepartment of Genome Sciences, University of Washington, Seattle, Washington, USA.
Judit VillénDepartment of Genome Sciences, University of Washington, Seattle, Washington, USA.
Vladimir GelevFaculty of Chemistry and Pharmacy, Sofia University, Sofia, Bulgaria.
Ellis L ReinherzLaboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
Haribabu ArthanariDepartment of Cancer Biology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
Harvard University · USThermo Fisher Scientific (United States) · USSofia University "St. Kliment Ohridski" · BGUniversity of Washington · USDana-Farber Cancer Institute · US

Funding

Protein Production CoreP01AI143565 · NIAID · DANA-FARBER CANCER INST · PI Matthew J. Lang, ELLIS L REINHERZ · 2020 to 2026
$19.9M
TRD3 NMRbox: Bayesian AnalyticsP41GM111135 · NIGMS · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI HOCH, JEFFREY C · 2015 to 2024
$14.0M
NIAID NIH HHS P01 AI143565NIGMS NIH HHS P41 GM111135NIH HHS P01AI143565NIH HHS P41GM111135
6 · The paper itself

Abstract

Protein nuclear magnetic resonance (NMR) spectroscopy relies on the ability to isotopically label polypeptides, which is achieved through heterologous expression in various host organisms. Most commonly, Escherichia coli is employed by leveraging isotopically substituted ammonium and glucose to uniformly label proteins with

Indexed as

Amino AcidsEscherichia coliAnimalsHumansMagnetic Resonance SpectroscopyMammalsNuclear Magnetic Resonance, BiomolecularReceptors, Antigen, T-CellAmino AcidsReceptors, Antigen, T-Cell19F‐labelingExpi293Fglycosylationlocal deuterationmammalian expressionmethyl TROSYnuclear magnetic resonance spectroscopy (NMR)preT‐cell receptor (preTCR)T‐cell receptor (TCR)

Identifiers

PMID38511503
PMCPMC10955624
OpenAlexW4393045212

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.