Evidence map›Paper›PMID 38510172›Full record

ArticleBiological imaging2023

TrackAnalyzer: A Fiji/ImageJ toolbox for a holistic analysis of tracks.

Ana Cayuela López, Eva M García-Cuesta, Sofía R Gardeta, José Miguel Rodríguez-Frade, Mario Mellado, José Antonio Gómez-Pedrero, Carlos Oscar S Sorzano

Abstract read
In one paragraph

Article in Biological imaging, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Ana Cayuela LópezBiocomputing Unit, National Centre for Biotechnology, Cantoblanco, Madrid, Spain.ORCID https://orcid.org/0000-0001-7728-9867
Eva M García-CuestaDepartment of Immunology and Oncology, National Centre for Biotechnology, Cantoblanco, Madrid, Spain.
Sofía R GardetaDepartment of Immunology and Oncology, National Centre for Biotechnology, Cantoblanco, Madrid, Spain.
José Miguel Rodríguez-FradeDepartment of Immunology and Oncology, National Centre for Biotechnology, Cantoblanco, Madrid, Spain.
Mario MelladoDepartment of Immunology and Oncology, National Centre for Biotechnology, Cantoblanco, Madrid, Spain.
José Antonio Gómez-PedreroApplied Optics Complutense Group, Faculty of Optics and Optometry, University Complutense of Madrid, Madrid, Spain.
Carlos Oscar S SorzanoBiocomputing Unit, National Centre for Biotechnology, Cantoblanco, Madrid, Spain.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Current live-cell imaging techniques make possible the observation of live events and the acquisition of large datasets to characterize the different parameters of the visualized events. They provide new insights into the dynamics of biological processes with unprecedented spatial and temporal resolutions. Here we describe the implementation and application of a new tool called TrackAnalyzer, accessible from Fiji and ImageJ. Our tool allows running semi-automated single-particle tracking (SPT) and subsequent motion classification, as well as quantitative analysis of diffusion and intensity for selected tracks relying on the graphical user interface (GUI) for large sets of temporal images (X-Y-T or X-Y-C-T dimensions). TrackAnalyzer also allows 3D visualization of the results as overlays of either spots, cells or end-tracks over time, along with corresponding feature extraction and further classification according to user criteria. Our analysis workflow automates the following steps: (1) spot or cell detection and filtering, (2) construction of tracks, (3) track classification and analysis (diffusion and chemotaxis), and (4) detailed analysis and visualization of all the outputs along the pipeline. All these analyses are automated and can be run in batch mode for a set of similar acquisitions.

Indexed as

celldiffusionsingle-particle trackingspottime-lapsetrack

Identifiers

PMID38510172
PMCPMC10951927

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.