ArticleBMC plant biology2024
Genome-wide characterization of the bHLH gene family in Gynostemma pentaphyllum reveals its potential role in the regulation of gypenoside biosynthesis.
Article in BMC plant biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed, 12 citations in OpenAlex.
- Genome-wide identification and functional characterization of bHLH transcription factors in Herpetospermum pedunculosum under salt stress.BMC plant biology · 2026Article
- Transcriptome-wide analysis of AlbHLH gene family in Atractylodes lancea (Thunb.) DC and functional exploration of AlbHLH3 in regulating terpenoid biosynthesis.BMC plant biology · 2025Article
- Identification, characterization, and expression analysis of WRKY transcription factors inFrontiers in plant science · 2025Article
- Identification of bHLH transcription factors and screening of anthocyanin-related genes in Lagerstroemia indica.Genetica · 2024Article
- Comprehensive Genome-Wide Identification and Expression Profiling ofInternational journal of molecular sciences · 2024Article
- Genome-Wide Identification of the bHLH Gene Family inInternational journal of molecular sciences · 2024Article
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9 authors at 1 institution in 1 country.
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Abstract
backgroundGynostemma pentaphyllum, an ancient Chinese herbal medicine, serves as a natural source of gypenosides with significant medicinal properties. Basic helix-loop-helix (bHLH) transcription factors play pivotal roles in numerous biological processes, especially in the regulation of secondary metabolism in plants. However, the characteristics and functions of the bHLH genes in G. pentaphyllum remain unexplored, and their regulatory role in gypenoside biosynthesis remains poorly elucidated.
resultsThis study identified a total of 111 bHLH members in G. pentaphyllum (GpbHLHs), categorizing them into 26 subgroups based on shared conserved motif compositions and gene structures. Collinearity analysis illustrated that segmental duplications predominately lead to the evolution of GpbHLHs, with most duplicated GpbHLH gene pairs undergoing purifying selection. Among the nine gypenoside-related GpbHLH genes, two GpbHLHs (GpbHLH15 and GpbHLH58) were selected for further investigation based on co-expression analysis and functional prediction. The expression of these two selected GpbHLHs was dramatically induced by methyl jasmonate, and their nuclear localization was confirmed. Furthermore, yeast one-hybrid and dual-luciferase assays demonstrated that GpbHLH15 and GpbHLH58 could bind to the promoters of the gypenoside biosynthesis pathway genes, such as GpFPS1, GpSS1, and GpOSC1, and activate their promoter activity to varying degrees.
conclusionsIn conclusion, our findings provide a detailed analysis of the bHLH family and valuable insights into the potential use of GpbHLHs to enhance the accumulation of gypenosides in G. pentaphyllum.
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