Evidence map›Paper›PMID 38507207›Full record

ArticleAdvances in experimental medicine and biology2024

Sample Preparation for Electron Cryo-Microscopy of Macromolecular Machines.

Aurélien Deniaud, Burak V Kabasakal, Joshua C Bufton, Christiane Schaffitzel

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In one paragraph

Article in Advances in experimental medicine and biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
3.0field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 3 countries.

Aurélien DeniaudUniv. Grenoble Alpes, CNRS, CEA, IRIG - Laboratoire de Chimie et Biologie des Métaux, Grenoble, France.
Burak V KabasakalSchool of Biochemistry, University of Bristol, Bristol, UK.
Joshua C BuftonSchool of Biochemistry, University of Bristol, Bristol, UK.
Christiane SchaffitzelSchool of Biochemistry, University of Bristol, Bristol, UK. christiane.berger-schaffitzel@bristol.ac.uk.
University of Bristol · GBCEA Grenoble · FRTurkish Society of Cardiology · TR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

High-resolution structure determination by electron cryo-microscopy underwent a step change in recent years. This now allows study of challenging samples which previously were inaccessible for structure determination, including membrane proteins. These developments shift the focus in the field to the next bottlenecks which are high-quality sample preparations. While the amounts of sample required for cryo-EM are relatively small, sample quality is the key challenge. Sample quality is influenced by the stability of complexes which depends on buffer composition, inherent flexibility of the sample, and the method of solubilization from the membrane for membrane proteins. It further depends on the choice of sample support, grid pre-treatment and cryo-grid freezing protocol. Here, we discuss various widely applicable approaches to improve sample quality for structural analysis by cryo-EM.

Indexed as

ElectronsMembrane ProteinsCryoelectron MicroscopyFreezingMacromolecular SubstancesSpecimen HandlingMacromolecular SubstancesMembrane ProteinsElectron cryo-microscopyGrid preparationMembrane proteinsSample optimizationSample preparationSample stability

Identifiers

PMID38507207
OpenAlexW4393009343

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.