ReviewBiophysics reviews2022
Live cell microscopy: From image to insight.
Review in Biophysics reviews, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- In the absence of mitochondrial fusion unequal segregation of mitochondria drives mtDNA loss.EMBO reports · 2026Article
- Diverse intracellular trafficking of insulin analogs by machine learning-based colocalization and diffusion analysis.iScience · 2026Article
- Imaging malaria parasites across scales and time.Journal of microscopy · 2026Review
- PhotoFiTT: a quantitative framework for assessing phototoxicity in live-cell microscopy experiments.Nature communications · 2025Article
- Discriminating single-molecule binding events from diffraction-limited fluorescence.Nature communications · 2025Article
- Live-cell painting: Image-based profiling in live cells using acridine orange.Molecular biology of the cell · 2025Article
- Video microscopy: an old story with a bright biological future.Biomedical engineering online · 2025Review
- Quantifying microbial robustness in dynamic environments using microfluidic single-cell cultivation.Microbial cell factories · 2024Article
- Establishment of the microscope incubation system and its application in evaluating tumor treatment effects through real-time live cellular imaging.Frontiers in bioengineering and biotechnology · 2024Article
- Comprehensive and quantitative analysis of intracellular structure polarization at the apical-basal axis in elongating Arabidopsis zygotes.Scientific reports · 2023Article
- Protocol for multispectral imaging on cryosections to map myeloid cell heterogeneity in its spatial context.STAR protocols · 2023Article
- Imagining the future of optical microscopy: everything, everywhere, all at once.Communications biology · 2023Review
- Protocol to perform dynamic microfluidic single-cell cultivation of C. glutamicum.STAR protocols · 2023Article
- Light-sheet autofluorescence lifetime imaging with a single-photon avalanche diode array.Journal of biomedical optics · 2023Article
- An optimized reporter of the transcription factor hypoxia-inducible factor 1α reveals complex HIF-1α activation dynamics in single cells.The Journal of biological chemistry · 2023Article
- Light sheet autofluorescence lifetime imaging with a single photon avalanche diode array.bioRxiv : the preprint server for biology · 2023Article
- Quality assessment in light microscopy for routine use through simple tools and robust metrics.The Journal of cell biology · 2022Article
- Segmentation, tracking and cell cycle analysis of live-cell imaging data with Cell-ACDC.BMC biology · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Live-cell microscopy is a powerful tool that can reveal cellular behavior as well as the underlying molecular processes. A key advantage of microscopy is that by visualizing biological processes, it can provide direct insights. Nevertheless, live-cell imaging can be technically challenging and prone to artifacts. For a successful experiment, many careful decisions are required at all steps from hardware selection to downstream image analysis. Facing these questions can be particularly intimidating due to the requirement for expertise in multiple disciplines, ranging from optics, biophysics, and programming to cell biology. In this review, we aim to summarize the key points that need to be considered when setting up and analyzing a live-cell imaging experiment. While we put a particular focus on yeast, many of the concepts discussed are applicable also to other organisms. In addition, we discuss reporting and data sharing strategies that we think are critical to improve reproducibility in the field.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.