Evidence map›Paper›PMID 38505002›Full record

ArticleAngewandte Chemie (Weinheim an der Bergstrasse, Germany)2022

Transmembrane Shuttling of Photosynthetically Produced Electrons to Propel Extracellular Biocatalytic Redox Reactions in a Modular Fashion.

Valentina Jurkaš, Florian Weissensteiner, Piera De Santis, Stephan Vrabl, Frieda A Sorgenfrei, Sarah Bierbaumer, Selin Kara, Robert Kourist, Pramod P Wangikar, Christoph K Winkler and 1 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Angewandte Chemie (Weinheim an der Bergstrasse, Germany), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.3field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 3 countries.

Valentina JurkašInstitute of Chemistry University of Graz Heinrichstraße 28 8010 Graz Austria.
Florian WeissensteinerInstitute of Chemistry University of Graz Heinrichstraße 28 8010 Graz Austria.
Piera De SantisInstitute of Chemistry University of Graz Heinrichstraße 28 8010 Graz Austria.
Stephan VrablInstitute of Chemistry University of Graz Heinrichstraße 28 8010 Graz Austria.
Frieda A SorgenfreiAustrian Centre of Industrial Biotechnology, c/o Institute of Chemistry, University of Graz Heinrichstraße 28 8010 Graz Austria.
Sarah BierbaumerInstitute of Chemistry University of Graz Heinrichstraße 28 8010 Graz Austria.
Selin KaraDepartment of Engineering, Biological and Chemical Engineering Section Biocatalysis and Bioprocessing Group Aarhus University Gustav Wieds Vej 10 8000 Aarhus Denmark.
Robert KouristInstitute of Molecular Biotechnology Graz University of Technology Petersgasse 14 8010 Graz Austria.
Pramod P WangikarDepartment of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076 India DBT-Pan IIT Centre for Bioenergy, Indian Institute of Technology Bombay, Powai, Mumbai 400076 India Wadhwani Research Centre for Bioengineering Indian Institute of Technology Bombay Powai Mumbai 400076 India.
Christoph K WinklerInstitute of Chemistry University of Graz Heinrichstraße 28 8010 Graz Austria.
Wolfgang KroutilInstitute of Chemistry University of Graz Heinrichstraße 28 8010 Graz Austria.ORCID 0000-0002-2151-6394
University of Graz · ATAarhus University · DKIndian Institute of Technology Bombay · INInstitute of Molecular Biotechnology · AT

Funding

Austrian Science Fund FWF DOC 46
6 · The paper itself

Abstract

Many biocatalytic redox reactions depend on the cofactor NAD(P)H, which may be provided by dedicated recycling systems. Exploiting light and water for NADPH-regeneration as it is performed, e.g. by cyanobacteria, is conceptually very appealing due to its high atom economy. However, the current use of cyanobacteria is limited, e.g. by challenging and time-consuming heterologous enzyme expression in cyanobacteria as well as limitations of substrate or product transport through the cell wall. Here we establish a transmembrane electron shuttling system propelled by the cyanobacterial photosynthesis to drive extracellular NAD(P)H-dependent redox reactions. The modular photo-electron shuttling (MPS) overcomes the need for cloning and problems associated with enzyme- or substrate-toxicity and substrate uptake. The MPS was demonstrated on four classes of enzymes with 19 enzymes and various types of substrates, reaching conversions of up to 99 % and giving products with >99 % optical purity.

Indexed as

BiocatalysisPhotocatalysisRedox ChemistryReductionsTransmembrane Shuttling

Identifiers

PMID38505002
PMCPMC10946770
OpenAlexW4289637344

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.