ArticleNature microbiology2024
Chromosomal barcodes for simultaneous tracking of near-isogenic bacterial strains in plant microbiota.
Article in Nature microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Engineering rhizobacterial communities for soil and plant health.Microbiology (Reading, England) · 2026Review
- Chromoprotein-modified plant pathogenic bacteria: tools for experimental tracking and visualization.Microbiology spectrum · 2026Article
- Review
- Upregulated jasmonate signaling shifts Arabidopsis microbiota interactions and stress adaptations through a positive feedback loop.The ISME journal · 2026Article
- Soil-borne diseases in medicinal plants: linking allelopathy and host immunity to microbiome-based interventions.Frontiers in plant science · 2026Review
- Quantifying host-microbe interactions with bacterial lineage tracing.Science (New York, N.Y.) · 2026Review
- Microbial SynComs as biocontrol tools: modulating plant immunity and future perspectives.Frontiers in microbiology · 2026Article
- Applying ecological principles to microbiome engineering.Nature microbiology · 2025Review
- Evaluation of Engineering Potential in Undomesticated Microbes With VECTOR.Microbial biotechnology · 2025Article
- Building microbial synthetic communities: get inspired by the design of synthetic plant communities.The New phytologist · 2025Article
- Conserved immunomodulation and variation in host association by Xanthomonadales commensals in Arabidopsis root microbiota.Nature plants · 2025Article
- Microbial diversity and function in bamboo ecosystems.Frontiers in microbiology · 2025Review
- A cross-systems primer for synthetic microbial communities.Nature microbiology · 2024Review
- Community standards and future opportunities for synthetic communities in plant-microbiota research.Nature microbiology · 2024Review
- Bacterial barcoding facilitates plant microbiome studies.Nature reviews. Microbiology · 2024Article
Corrections and comments
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Authors and funding
8 authors at 7 institutions in 7 countries.
Funding
Abstract
DNA-amplicon-based microbiota profiling can estimate species diversity and abundance but cannot resolve genetic differences within individuals of the same species. Here we report the development of modular bacterial tags (MoBacTags) encoding DNA barcodes that enable tracking of near-isogenic bacterial commensals in an array of complex microbiome communities. Chromosomally integrated DNA barcodes are then co-amplified with endogenous marker genes of the community by integrating corresponding primer binding sites into the barcode. We use this approach to assess the contributions of individual bacterial genes to Arabidopsis thaliana root microbiota establishment with synthetic communities that include MoBacTag-labelled strains of Pseudomonas capeferrum. Results show reduced root colonization for certain mutant strains with defects in gluconic-acid-mediated host immunosuppression, which would not be detected with traditional amplicon sequencing. Our work illustrates how MoBacTags can be applied to assess scaling of individual bacterial genetic determinants in the plant microbiota.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.