Evidence map›Paper›PMID 38502469›Full record

ArticleOdontology2024

Synthesis of secretory leukocyte protease inhibitor using cell-free protein synthesis system.

Yuka Hiroshima, Rie Kido, Jun-Ichi Kido, Mika Bando, Kaya Yoshida, Akikazu Murakami, Yasuo Shinohara

Abstract read
PubMed Publisher
In one paragraph

Article in Odontology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Yuka HiroshimaDepartment of Oral Microbiology, Tokushima University Graduate School of Biomedical Sciences, 3-18-15, Kuramoto, Tokushima, 770-8504, Japan. yuka.hiroshima@tokushima-u.ac.jp.ORCID http://orcid.org/0000-0003-3175-7850
Rie KidoDepartment of Periodontology and Endodontology, Tokushima University Graduate School of Biomedical Sciences, Tokushima, Japan.ORCID http://orcid.org/0000-0001-9043-5208
Jun-Ichi KidoDepartment of Periodontology and Endodontology, Tokushima University Graduate School of Biomedical Sciences, Tokushima, Japan.ORCID http://orcid.org/0000-0002-3949-5931
Mika BandoDepartment of Periodontology and Endodontology, Tokushima University Graduate School of Biomedical Sciences, Tokushima, Japan.ORCID http://orcid.org/0000-0003-3996-3595
Kaya YoshidaDepartment of Oral Healthcare Promotion, Tokushima University Graduate School of Biomedical Sciences, Tokushima, Japan.ORCID http://orcid.org/0000-0002-3202-0634
Akikazu MurakamiDepartment of Oral Microbiology, Tokushima University Graduate School of Biomedical Sciences, 3-18-15, Kuramoto, Tokushima, 770-8504, Japan.ORCID http://orcid.org/0000-0003-3064-3237
Yasuo ShinoharaInstitute of Advanced Medical Sciences, Tokushima University, Tokushima, Japan.ORCID http://orcid.org/0000-0002-5331-3508
Tokushima University · JP

Funding

Japan Society for the Promotion of Science 17H04418Japan Society for the Promotion of Science 20K09941Japan Society for the Promotion of Science 20K23083Japan Society for the Promotion of Science 22K17040
6 · The paper itself

Abstract

Secretory leukocyte protease inhibitor (SLPI) functions as a protease inhibitor that modulates excessive proteolysis in the body, exhibits broad-spectrum antimicrobial activity, regulates inflammatory responses, and plays an important role in the innate immunity. The purpose of the study was to artificially synthesize a SLPI, an antimicrobial peptide, and investigate its effect on antimicrobial activity against Porphyromonas gingivalis and interleukin-6 (IL-6) production. SLPI protein with a molecular weight of approximately 13 kDa was artificially synthesized using a cell-free protein synthesis (CFPS) system and investigated by western blotting and enzyme-linked immunosorbent assay (ELISA). Disulfide bond isomerase in the protein synthesis mixture increased the amount of SLPI synthesized. The synthesized SLPI (sSLPI) protein was purified and its antimicrobial activity was investigated based on the growth of Porphyromonas gingivalis and bacterial adhesion to oral epithelial cells. The effect of sSLPI on IL-6 production in human periodontal ligament fibroblasts (HPLFs) was examined by ELISA. Our results showed that sSLPI significantly inhibited the growth of Porphyromonas gingivalis and bacterial adhesion to oral epithelial cells and further inhibited IL-6 production by HPLFs. These results suggested that SLPI artificially synthesized using the CFPS system may play a role in the prevention of periodontal diseases through its antimicrobial and anti-inflammatory effects.

Indexed as

Cell-Free SystemEnzyme-Linked Immunosorbent AssayInterleukin-6Porphyromonas gingivalisSecretory Leukocyte Peptidase InhibitorBlotting, WesternCells, CulturedEpithelial CellsFibroblastsHumansPeriodontal LigamentInterleukin-6Secretory Leukocyte Peptidase InhibitorAntimicrobial activityCell-free protein synthesis systemInterleukin-6Porphyromonas gingivalisSecretory leukocyte protease inhibitor

Identifiers

PMID38502469
OpenAlexW4392960050

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.