ArticleMethods in molecular biology (Clifton, N.J.)2025
Principles of Indirect Co-culture Method Using Transwell.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 14 citations in OpenAlex.
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- Induced Pluripotent Stem Cell-Based Platforms for Cardiac-Related Pain Research: Molecular Mechanisms, Experimental Models, and Therapeutic Applications.International journal of molecular sciences · 2026Review
- Supercritical COMaterials today. Bio · 2026Article
- Engineering a Compartmentalized Multi-Cell Co-Culture Hydrogel System Using Beeswax/Fucoidan/Alginate for Cultured Meat Modeling.Foods (Basel, Switzerland) · 2026Article
- Modeling blood-brain barrier-glioblastoma interactions: implications for chemoresistance and therapeutic targeting.Fluids and barriers of the CNS · 2026Review
- Engineering osteoporosis-related bone organoids: a mechanism-module-functional readout framework for disease modelling and biomarker translation.Frontiers in cell and developmental biology · 2026Review
- Mechanistic Insights into the Role of Extracellular Vesicles at the Bacterial-Host Interface.ACS infectious diseases · 2025Article
- Pre-clinical evaluation of mRNA-lipid nanoparticles' potency and toxicity: current practices and future directions.In vitro models · 2025Review
- Emerging advances in intestinal models for in vitro preclinical research.American journal of physiology. Gastrointestinal and liver physiology · 2025Review
- CCL2-CCR2 Axis Inhibition in Osteosarcoma Cell Model: The Impact of Oxygen Level on Cell Phenotype.Journal of cellular physiology · 2025Article
- Synergistic Ion-Releasing Nanoparticles as a Therapeutic Platform for Modulating Adult Stem Cell Activity in Wound Healing.Biomaterials research · 2025Article
- Harnessing 3D models to uncover the mechanisms driving infectious and inflammatory disease in the intestine.BMC biology · 2024Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The co-culture method is a simple type of cell culture method used to evaluate the effects of communication between various types of cells in an in vitro setting. In the co-culture method, two or more eukaryotic cell types, or eukaryotic and prokaryotic cells, are cultured together. The co-culture method reflects in vivo cell behaviors and thereby emerges as a pivotal technique with diverse applications in cancer research and cell biology. Two categories of co-culture methods (indirect methods and direct methods) are well known. Direct co-culture methods allow physical contact between the various cell types (juxtacrine signaling). In indirect methods, cells are physically separated into two different populations (for example, using a Transwell) that allow communication only via secretory factors (paracrine signaling). Herein, we focus on the principles of the indirect co-culture method. Nowadays, this method is used to explore the effects of mesenchymal stem cell (MSC) secretome on cancer cells. These studies have unveiled intricate cell behavior dynamics, demonstrating how the MSC secretome influences cancer cell proliferation, invasion, apoptosis, and polarity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.