Evidence map›Paper›PMID 38502253›Full record

ArticleArchives of microbiology2024

Molecular cloning, expression, purification, and characterization of Bacillus subtilis hydrolyzed ginsenoside Rc of α-L-arabinofuranosidase in Escherichia coli.

Ling Zhu, Yuchen Wang, Jian Cai

Abstract read
PubMed Publisher
In one paragraph

Article in Archives of microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Ling ZhuYunnan Engineering Research Center of Fruit Wine, Qujing Normal University, Qujing, 655011, People's Republic of China.
Yuchen WangSchool of Chemical Biology and Environment, Yuxi Normal University, Yuxi, 653100, People's Republic of China. ycwang@yxnu.edu.cn.
Jian CaiYunnan Engineering Research Center of Fruit Wine, Qujing Normal University, Qujing, 655011, People's Republic of China. caijian928@outlook.com.
Qujing Normal University · CNYuxi Normal University · CN

Funding

Special Basic Cooperative Research Innovation Programs of Qujing Science and Technology Bureau & Qujing Normal University KJLH2022YB07Special Basic Cooperative Research Innovation Programs of Qujing Science and Technology Bureau & Qujing Normal University, KJLH2023ZD06Special Basic Cooperative Research Programs of Yunnan Provincial Undergraduate Universities' Association 202301BA070001-081Yunnan Fundamental Research Projects 202301AU070012
6 · The paper itself

Abstract

The α-L-arabinofuranosidase enzyme plays a crucial role in the degradation of ginsenosides. In this study, we successfully cloned and expressed a novel α-L-arabinofuranosidase bsafs gene (1503 bp, 501 amino acids, 55 kDa, and pI = 5.4) belonging to glycosyl hydrolase (GH) family 51 from Bacillus subtilis genome in Escherichia coli BL21 cells. The recombinant protein Bsafs was purified using Ni

Indexed as

GinsenosidesBacillus subtilisCloning, MolecularEscherichia coliGlycoside HydrolasesRecombinant ProteinsSubstrate Specificityalpha-N-arabinofuranosidaseginsenoside RcGinsenosidesGlycoside HydrolasesRecombinant ProteinsBacillus subtilisBiotransformationGinsenoside RcGlycosyl hydrolase 51α-L-arabinofuranosidase

Identifiers

PMID38502253
OpenAlexW4392952353

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.