Evidence map›Paper›PMID 38499918›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Evaluation of Autophagy in Conjunctival Fibroblasts.

Parvaneh Mehrbod, Paola Brun, Umberto Rosani, Andrea Leonardi, Saeid Ghavami

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.6field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 4 countries.

Parvaneh MehrbodDepartment of Influenza and Respiratory Viruses, Pasteur Institute of Iran, Tehran, Iran.
Paola BrunDepartment of Molecular Medicine, Histology Unit, University of Padova, Padua, Italy.
Umberto RosaniDepartment of Biology, University of Padova, Padova, Italy.
Andrea LeonardiDepartment of Neuroscience, Ophthalmology Unit, University of Padova, Padua, Italy.
Saeid GhavamiBiology of Breathing Theme, Children Hospital Research Institute of Manitoba, University of Manitoba, Winnipeg, MB, Canada. saeid.ghavami@umanitoba.ca.
University of Padua · ITCancerCare Manitoba · CAPasteur Institute of Iran · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Vernal keratoconjunctivitis (VKC) is a serious eye allergy characterized by poorly understood pathogenic mechanisms and a lack of effective treatments. Autophagy, a process involved in both triggering and suppressing immune and inflammatory responses, plays a role in VKC's pathophysiology. Understanding autophagy's involvement in VKC could lead to new treatment possibilities, such as utilizing specific topical substances to induce or inhibit autophagy and prevent severe complications of this eye condition. In our current protocol, we present a robust methodology established in our laboratory for studying autophagy in primary conjunctival fibroblasts. We assess autophagy through techniques like immunocytochemistry, immunoblotting, and qPCR.

Indexed as

AutophagyConjunctivaConjunctivitis, AllergicFibroblastsCells, CulturedHumansImmunoblottingImmunohistochemistryReal-Time Polymerase Chain ReactionAutophagy fluxChloroquineVernal keratoconjunctivitis

Identifiers

PMID38499918
OpenAlexW4392931528

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.