Evidence map›Paper›PMID 38499840›Full record

ArticleMikrochimica acta2024

Ultrasensitive colorimetric detection of Staphylococcus aureus using wheat germ agglutinin and IgY as a dual-recognition strategy.

Yun Zhang, Ge Tian, Xueni Sun, Xiaoli Yang, Yi Zhang, Wenqing Tan, Liangwei Duan, Shunxiang Gao, Junping Yu

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.5field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 8 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Yun ZhangLaboratory of Infection and Immunology, School of Medical Technology, Xinxiang Medical University, Xinxiang, 453003, People's Republic of China.
Ge TianLaboratory of Infection and Immunology, School of Medical Technology, Xinxiang Medical University, Xinxiang, 453003, People's Republic of China.
Xueni SunLaboratory of Infection and Immunology, School of Medical Technology, Xinxiang Medical University, Xinxiang, 453003, People's Republic of China.
Xiaoli YangLaboratory of Infection and Immunology, School of Medical Technology, Xinxiang Medical University, Xinxiang, 453003, People's Republic of China.
Yi ZhangLaboratory of Infection and Immunology, School of Medical Technology, Xinxiang Medical University, Xinxiang, 453003, People's Republic of China.
Wenqing TanLaboratory of Infection and Immunology, School of Medical Technology, Xinxiang Medical University, Xinxiang, 453003, People's Republic of China.
Liangwei DuanLaboratory of Infection and Immunology, School of Medical Technology, Xinxiang Medical University, Xinxiang, 453003, People's Republic of China. duanliangwei@xxmu.edu.cn.
Shunxiang GaoDepartment of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, People's Republic of China. shunxianggao@163.com.
Junping YuLaboratory of Infection and Immunology, School of Medical Technology, Xinxiang Medical University, Xinxiang, 453003, People's Republic of China. yujp@wh.iov.cn.ORCID http://orcid.org/0000-0001-9024-0793
Xinxiang Medical University · CNChinese Academy of Sciences · CNShanghai Jiao Tong University · CN

Funding

Key Scientific Research Projects of Higher Education Institutions in Henan Province 23A330004Key Scientific Research Projects of Key Scientific and Technological Projects of Henan Province 222102310310Key Scientific Research Projects of Key Scientific and Technological Projects of Henan Province 222102310476the National Natural Science Foundation of China 81702099
6 · The paper itself

Abstract

A novel colorimetric platform was designed for the determination of S. aureus by utilizing a dual-recognition strategy, where wheat germ agglutinin (WGA)-functionalized magnetic beads were served as separation elements to capture and enrich S. aureus efficiently from the matrix. Horseradish peroxidase (HRP) labeled chicken anti-protein A IgY (HRP-IgY) was used to label the captured S. aureus. A chicken IgY was introduced as a signal tracer to bind with staphylococcal protein A (SPA) on the surface of S. aureus, which can circumvent the interference from protein G-producing Streptococcus. Subsequently, the colorimetric signal was achieved by an HRP-catalyzed reaction, which was amplified by HRP-IgY bound by approximately 80,000 SPA molecules on one S. aureus. The entire detection process could be accomplished within 90 min. Under optimal conditions, the linear response of different S. aureus concentrations ranged from 7.8 × 10

Indexed as

Staphylococcal InfectionsStaphylococcus aureusColorimetryHorseradish PeroxidaseHumansImmunoglobulinsWheat Germ AgglutininsHorseradish PeroxidaseIgYImmunoglobulinsWheat Germ AgglutininsChicken IgYS. aureusSpecificityWheat germ agglutinin

Identifiers

PMID38499840
OpenAlexW4392968708

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.