Evidence map›Paper›PMID 38499730›Full record

ArticleWorld journal of microbiology & biotechnology2024

Rice N-biofertilization by inoculation with an engineered photosynthetic diazotroph.

Yan Zeng, Mengmei Wang, Yunkai Yu, Lida Wang, Lingwei Cui, Chang Li, Ying Liu, Yanning Zheng

Abstract read
PubMed Publisher
In one paragraph

Article in World journal of microbiology & biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. PApplied and environmental microbiology · 2025
    Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

Yan Zeng *State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No.1 Beichen West Road, Chaoyang District, Beijing, 100101, China.
Mengmei Wang *State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No.1 Beichen West Road, Chaoyang District, Beijing, 100101, China.
Yunkai YuQiqihar Branch of Heilongjiang Academy of Agricultural Sciences, Qiqihar, 161006, China.
Lida WangQiqihar Branch of Heilongjiang Academy of Agricultural Sciences, Qiqihar, 161006, China.
Lingwei CuiState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No.1 Beichen West Road, Chaoyang District, Beijing, 100101, China.
Chang LiState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No.1 Beichen West Road, Chaoyang District, Beijing, 100101, China.
Ying LiuState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No.1 Beichen West Road, Chaoyang District, Beijing, 100101, China.
Yanning ZhengState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No.1 Beichen West Road, Chaoyang District, Beijing, 100101, China. zhengyn@im.ac.cn.
Institute of Microbiology · CNHeilongjiang Provincial Academy of Agricultural Sciences · CNShanxi Agricultural University · CNUniversity of Chinese Academy of Sciences · CN

Funding

National Key Research and Development Program of China 2020YFA0906800National Key Research and Development Program of China 2022YFC2105300National Natural Science Foundation of China 32300032National Natural Science Foundation of China 91851102Strategic Priority Research Program of the Chinese Academy of Sciences XDA28030201
6 · The paper itself

Abstract

Photosynthetic diazotrophs expressing iron-only (Fe-only) nitrogenase can be developed into a promising biofertilizer, as it is independent on the molybdenum availability in the soil. However, the expression of Fe-only nitrogenase in diazotrophs is repressed by the fixed nitrogen of the soil, limiting the efficiency of nitrogen fixation in farmland with low ammonium concentrations that are inadequate for sustainable crop growth. Here, we succeeded in constitutively expressing the Fe-only nitrogenase even in the presence of ammonium by controlling the transcription of Fe-only nitrogenase gene cluster (anfHDGK) with the transcriptional activator of Mo nitrogenase (NifA*) in several different ways, indicating that the engineered NifA* strains can be used as promising chassis cells for efficient expression of different types of nitrogenases. When applied as a biofertilizer, the engineered Rhodopseudomonas palustris effectively stimulated rice growth, contributing to the reduced use of chemical fertilizer and the development of sustainable agriculture.

Indexed as

Ammonium CompoundsOryzaNitrogenNitrogenaseNitrogen FixationSoilAmmonium CompoundsNitrogenNitrogenaseSoilBiofertilizerConstitutive expressionFe-only nitrogenasePhotosynthetic diazotrophSustainable agriculture

Identifiers

PMID38499730
OpenAlexW4392968704

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.