Evidence map›Paper›PMID 38498603›Full record

ArticleAngewandte Chemie (International ed. in English)2024

Metabolic-Glycoengineering-Enabled Molecularly Specific Acoustic Tweezing Cytometry for Targeted Mechanical Stimulation of Cell Surface Sialoglycans.

Weiping Li, Jiatong Guo, Eric C Hobson, Xufeng Xue, Qingjiang Li, Jianping Fu, Cheri X Deng, Zhongwu Guo

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Angewandte Chemie (International ed. in English), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Weiping LiDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI-48109, USA.
Jiatong GuoDepartment of Chemistry, University of Florida, Gainesville, FL-32611, USA.
Eric C HobsonDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI-48109, USA.
Xufeng XueDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI-48109, USA.
Qingjiang LiDepartment of Chemistry, University of Florida, Gainesville, FL-32611, USA.
Jianping FuDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI-48109, USA.
Cheri X DengDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI-48109, USA.
Zhongwu GuoDepartment of Chemistry, University of Florida, Gainesville, FL-32611, USA.ORCID 0000-0001-5302-6456
University of Michigan · USUniversity of Florida · USUniversity of Florida Health · US

Funding

Synthetic and Biological Studies of GPI Conjugates and GPI Anchorage to Cell MembranesR35GM131686 · NIGMS · UNIVERSITY OF FLORIDA · PI Zhongwu Guo · 2019 to 2026
$2.5M
Acoustic Tweezing Cytometry for Efficient Neural DifferentiationR01GM143297 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DENG, CHERI X, FU, JIANPING · 2021 to 2024
$1.8M
NIGMS NIH HHS R01 GM143297NIGMS NIH HHS R35 GM131686
6 · The paper itself

Abstract

In this study, we developed a novel type of dibenzocyclooctyne (DBCO)-functionalized microbubbles (MBs) and validated their attachment to azide-labelled sialoglycans on human pluripotent stem cells (hPSCs) generated by metabolic glycoengineering (MGE). This enabled the application of mechanical forces to sialoglycans on hPSCs through molecularly specific acoustic tweezing cytometry (mATC), that is, displacing sialoglycan-anchored MBs using ultrasound (US). It was shown that subjected to the acoustic radiation forces of US pulses, sialoglycan-anchored MBs exhibited significantly larger displacements and faster, more complete recovery after each pulse than integrin-anchored MBs, indicating that sialoglycans are more stretchable and elastic than integrins on hPSCs in response to mechanical force. Furthermore, stimulating sialoglycans on hPSCs using mATC reduced stage-specific embryonic antigen-3 (SSEA-3) and GD3 expression but not OCT4 and SOX2 nuclear localization. Conversely, stimulating integrins decreased OCT4 nuclear localization but not SSEA-3 and GD3 expression, suggesting that mechanically stimulating sialoglycans and integrins initiated distinctive mechanoresponses during the early stages of hPSC differentiation. Taken together, these results demonstrated that MGE-enabled mATC uncovered not only different mechanical properties of sialoglycans on hPSCs and integrins but also their different mechanoregulatory impacts on hPSC differentiation, validating MGE-based mATC as a new, powerful tool for investigating the roles of glycans and other cell surface biomolecules in mechanotransduction.

Indexed as

Pluripotent Stem CellsHumansMetabolic EngineeringMicrobubblesAcoustic tweezing cytometryGlycoengineeringHuman pluripotent stem cellsMechanobiologySialoglycans

Identifiers

PMID38498603
PMCPMC11073901
OpenAlexW4392928399

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.