Evidence map›Paper›PMID 38497657›Full record

ArticleJournal of visualized experiments : JoVE2024

Two-Photon Intravital Microscopy of Glioblastoma in a Murine Model.

Kerem Nernekli, Dilyana B Mangarova, Yifeng Shi, Zahra Shokri Varniab, Edwin Chang, Oguz Ziya Tikenogullari, Laura Pisani, Grigory Tikhomirov, Gordon Wang, Heike E Daldrup-Link

Open access · greenAbstract readVideo-Audio Media
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of visualized experiments : JoVE, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 58% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Kerem Nernekli *Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University School of Medicine.
Dilyana B Mangarova *Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University School of Medicine; mangarod@stanford.edu.
Yifeng ShiDepartment of Electrical Engineering and Computer Sciences, University of California, Berkeley.
Zahra Shokri VarniabMolecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University School of Medicine.
Edwin ChangMolecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University School of Medicine.
Oguz Ziya TikenogullariDepartment of Mechanical Engineering, Stanford University.
Laura PisaniMolecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University School of Medicine.
Grigory TikhomirovDepartment of Electrical Engineering and Computer Sciences, University of California, Berkeley.
Gordon WangDepartment of Psychiatry and Behavioral Sciences, Stanford University, Wu Tsai Neuroscience Institute, Stanford University.
Heike E Daldrup-LinkMolecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University School of Medicine.
Stanford University · USUniversity of California, Berkeley · USNeuroscience Institute · IT

Funding

Theranostics for Pediatric Brain CancerR01HD103638 · NICHD · STANFORD UNIVERSITY · PI DALDRUP-LINK, HEIKE ELIZABETH · 2021 to 2025
$3.3M
Upgrade of the Stanford GE-Varian Experimental MRI Scanner to the Current Model MS10RR026917 · NCRR · STANFORD UNIVERSITY · PI MOSELEY, MICHAEL E · 2010 to 2010
$497k
NCRR NIH HHS S10 RR026917NICHD NIH HHS R01 HD103638
6 · The paper itself

Abstract

The delivery of intravenously administered cancer therapeutics to brain tumors is limited by the blood-brain barrier. A method to directly image the accumulation and distribution of macromolecules in brain tumors in vivo would greatly enhance our ability to understand and optimize drug delivery in preclinical models. This protocol describes a method for real-time in vivo tracking of intravenously administered fluorescent-labeled nanoparticles with two-photon intravital microscopy (2P-IVM) in a mouse model of glioblastoma (GBM). The protocol contains a multi-step description of the procedure, including anesthesia and analgesia of experimental animals, creating a cranial window, GBM cell implantation, placing a head bar, conducting 2P-IVM studies, and post-surgical care for long-term follow-up studies. We show representative 2P-IVM imaging sessions and image analysis, examine the advantages and disadvantages of this technology, and discuss potential applications. This method can be easily modified and adapted for different research questions in the field of in vivo preclinical brain imaging.

Indexed as

Brain NeoplasmsGlioblastomaAnimalsBrainDisease Models, AnimalIntravital MicroscopyMice

Identifiers

PMID38497657
PMCPMC11606332
OpenAlexW4392767603

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.