Evidence map›Paper›PMID 38497640›Full record

ArticleApplied and environmental microbiology2024

Combining CRISPR/Cas9 and natural excision for the precise and complete removal of mobile genetic elements in bacteria.

Pengxia Wang, Xiaofei Du, Yi Zhao, Weiquan Wang, Tongxuan Cai, Kaihao Tang, Xiaoxue Wang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied and environmental microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
3.7field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Efficient genetic manipulation ofApplied and environmental microbiology · 2025
    Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Pengxia Wang *Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Innovation Academy of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, China, Guangzhou.ORCID 0000-0002-1267-6118
Xiaofei Du *Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Innovation Academy of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, China, Guangzhou.
Yi ZhaoUniversity of Chinese Academy of Sciences, Beijing, China.
Weiquan WangKey Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Innovation Academy of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, China, Guangzhou.
Tongxuan CaiKey Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Innovation Academy of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, China, Guangzhou.
Kaihao TangKey Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Innovation Academy of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, China, Guangzhou.
Xiaoxue WangKey Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Innovation Academy of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, China, Guangzhou.ORCID 0000-0002-7257-1916
Chinese Academy of Sciences · CNUniversity of Chinese Academy of Sciences · CN

Funding

Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program 2019BT02Y262MOST | National Key Research and Development Program of China (NKPs) 2022YFC3103600MOST | National Natural Science Foundation of China (NSFC) 32070175MOST | National Natural Science Foundation of China (NSFC) 42188102MOST | National Natural Science Foundation of China (NSFC) 91951203Youth Innovation Promotion Association of the Chinese Academy of Sciences (CAS YIPA) 2021345
6 · The paper itself

Abstract

Horizontal gene transfer, facilitated by mobile genetic elements (MGEs), is an adaptive evolutionary process that contributes to the evolution of bacterial populations and infectious diseases. A variety of MGEs not only can integrate into the bacterial genome but also can survive or even replicate like plasmids in the cytoplasm, thus requiring precise and complete removal for studying their strategies in benefiting host cells. Existing methods for MGE removal, such as homologous recombination-based deletion and excisionase-based methods, have limitations in effectively eliminating certain MGEs. To overcome these limitations, we developed the Cas9-NE method, which combines the CRISPR/

Indexed as

CRISPR-Cas SystemsRNA, Guide, CRISPR-Cas SystemsBacteriaGene Transfer, HorizontalGenomic IslandsInterspersed Repetitive SequencesPlasmidsRNA, Guide, CRISPR-Cas SystemsCRISPR/Cas9excisiongenomic islandmobile genetic elements (MGEs)plasmidprophageVibrio

Identifiers

PMID38497640
PMCPMC11022536
OpenAlexW4392916820

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.