Evidence map›Paper›PMID 38497547›Full record

ArticleBiopreservation and biobanking2024

Technology for Biobanking of Epididymal Spermatozoa from Patient with Obstructive Azoospermia: Case Report about Baby Born after Conventional Freezing Only with a Nonpermeable Cryoprotectant 360 kDa Polyvinylpyrrolidone.

Maryna Petrushko, Taisiia Yurchuk, Volodymyr Piniaiev, Evgenia Isachenko, Gohar Rahimi, Peter Mallmann, Volodimir Isachenko

Open access · greenAbstract readCase Reports
PubMed Full text at PMC Publisher
In one paragraph

Article in Biopreservation and biobanking, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 94% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 3 countries.

Maryna PetrushkoInstitute for Problems of Cryobiology and Cryomedicine Nasu, Kharkiv, Ukraine.
Taisiia YurchukInstitute for Problems of Cryobiology and Cryomedicine Nasu, Kharkiv, Ukraine.ORCID 0000-0002-4993-9129
Volodymyr PiniaievInstitute for Problems of Cryobiology and Cryomedicine Nasu, Kharkiv, Ukraine.
Evgenia IsachenkoResearch Group for Reproductive Medicine, Department of Obstetrics and Gynecology, Medical Faculty, Cologne University, Cologne, Germany.
Gohar RahimiResearch Group for Reproductive Medicine, Department of Obstetrics and Gynecology, Medical Faculty, Cologne University, Cologne, Germany.
Peter MallmannResearch Group for Reproductive Medicine, Department of Obstetrics and Gynecology, Medical Faculty, Cologne University, Cologne, Germany.
Volodimir IsachenkoResearch Group for Reproductive Medicine, Department of Obstetrics and Gynecology, Medical Faculty, Cologne University, Cologne, Germany.ORCID 0000-0002-3674-543X
University of Cologne · DEInstitute for Problems of Cryobiology and Cryomedicine · UAInLife Institute of Animal Reproduction and Food Research, Polish Academy of Sciences · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This publication reports, for the first time, the birth of a healthy child after intracytoplasmic sperm injection (ICSI) of motile spermatozoa after conventional ("slow") freezing of epididymal spermatozoa using 5% polyvinylpyrrolidone (PVP) of high molecular weight (360 kDa). Cryopreservation solution with 10% PVP was added to 30 µL of spermatozoa suspension in a 1:1 ratio, with a final PVP concentration of 5%. Then, polycarbonate capillaries for oocyte denudation with a diameter of 170 µm were filled with 60 µL of the resulting sperm suspension. After that, the capillaries were placed for 10 minutes at a height of 15 cm above liquid nitrogen and immersed into liquid nitrogen. To warm the spermatozoa, the capillaries were immersed in a water bath at a temperature of 40°C for 30 seconds. Oocyte fertilization was performed by ICSI. Zygotes were cultured

Indexed as

AzoospermiaCryopreservationCryoprotective AgentsPovidoneSpermatozoaSperm Injections, IntracytoplasmicBiological Specimen BanksEpididymisFemaleFreezingHumansInfant, NewbornMalePregnancySemen PreservationCryoprotective AgentsPovidoneazoospermiababy borncryoprotectants-freeepididymalhuman spermatozoaslow freezing

Identifiers

PMID38497547
PMCPMC11656121
OpenAlexW4392913232

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.