Evidence map›Paper›PMID 38496645›Full record

ArticlebioRxiv : the preprint server for biology2024

A tale of two fusion proteins: understanding the metastability of human respiratory syncytial virus and metapneumovirus and implications for rational design of uncleaved prefusion-closed trimers.

Yi-Zong Lee, Jerome Han, Yi-Nan Zhang, Garrett Ward, Keegan Braz Gomes, Sarah Auclair, Robyn L Stanfield, Linling He, Ian A Wilson, Jiang Zhu

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Yi-Zong LeeDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
Jerome HanDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
Yi-Nan ZhangDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
Garrett WardDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
Keegan Braz GomesDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
Sarah AuclairDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
Robyn L StanfieldDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
Linling HeDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
Ian A WilsonDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
Jiang ZhuDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
Scripps Research Institute · US

Funding

A Synchrotron Radiation Structural Biology ResourcesP30GM133894 · NIGMS · STANFORD UNIVERSITY · PI Aina E. Cohen, KEITH O HODGSON · 2020 to 2026
$43.3M
NIGMS NIH HHS P30 GM133894
6 · The paper itself

Abstract

Respiratory syncytial virus (RSV) and human metapneumovirus (hMPV) cause human respiratory diseases and are major targets for vaccine development. In this study, we designed uncleaved prefusion-closed (UFC) trimers for the fusion (F) proteins of both viruses by examining mutations critical to F metastability. For RSV, we assessed four previous prefusion F designs, including the first and second generations of DS-Cav1, SC-TM, and 847A. We then identified key mutations that can maintain prefusion F in a native-like, closed trimeric form (up to 76%) without introducing any interprotomer disulfide bond. For hMPV, we developed a stable UFC trimer with a truncated F

Identifiers

PMID38496645
PMCPMC10942449
OpenAlexW4392624371

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.