Evidence map›Paper›PMID 38496594›Full record

ArticlebioRxiv : the preprint server for biology2024

Protein complex heterogeneity and topology revealed by electron capture charge reduction and surface induced dissociation.

Jared B Shaw, Sophie R Harvey, Chen Du, Zhixin Xu, Regina M Edgington, Eduardo Olmedillas, Erica Ollmann Saphire, Vicki H Wysocki

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Jared B ShawDepartment of Chemistry, University of Nebraska, Lincoln, NE 68588.ORCID 0000-0002-1130-1728
Sophie R HarveyNative Mass Spectrometry Guided Structural Biology Center, Ohio State University, Columbus, OH 43210.ORCID 0000-0003-0763-8173
Chen DuNative Mass Spectrometry Guided Structural Biology Center, Ohio State University, Columbus, OH 43210.ORCID 0000-0001-6161-5525
Zhixin XuNative Mass Spectrometry Guided Structural Biology Center, Ohio State University, Columbus, OH 43210.ORCID 0009-0008-1111-4042
Regina M EdgingtonDepartment of Chemistry and Biochemistry, Ohio State University, Columbus, OH 43210.
Eduardo OlmedillasCenter for Vaccine Innovation, La Jolla Institute for Immunology, La Jolla, CA 92037.ORCID 0000-0001-5250-0371
Erica Ollmann SaphireCenter for Vaccine Innovation, La Jolla Institute for Immunology, La Jolla, CA 92037.ORCID 0000-0002-1206-7451
Vicki H WysockiNative Mass Spectrometry Guided Structural Biology Center, Ohio State University, Columbus, OH 43210.ORCID 0000-0003-0495-2538
The Ohio State University · USLa Jolla Institute for Immunology · USUniversity of Nebraska–Lincoln · US

Funding

Targeted mass spectrometry approaches to understand CART processing and recepter interactionsP20GM113126 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI ZHOU, YOU · 2016 to 2025
$20.8M
Technology and Computational CoreP01AI165072 · NIAID · BRIGHAM AND WOMEN'S HOSPITAL · PI WESEMANN, DUANE R. · 2021 to 2023
$19.6M
Waters Select Series Cyclic IMS for P41 Native MS Resource Application to Alzheimer's DiseaseP41GM128577 · NIGMS · OHIO STATE UNIVERSITY · PI WYSOCKI, VICKI H. · 2018 to 2022
$7.6M
Native Mass Spectrometry Guided Structural Biology CenterRM1GM149374 · NIGMS · OHIO STATE UNIVERSITY · PI Vicki H. Wysocki · 2023 to 2026
$5.0M
Advancing native top-down protein analysis with hybrid SID/ECD technologyR43GM140749 · NIGMS · E-MSION, INC. · PI SHAW, JARED BRYAN · 2021 to 2021
$252k
NIAID NIH HHS P01 AI165072NIGMS NIH HHS P20 GM113126NIGMS NIH HHS P41 GM128577NIGMS NIH HHS R43 GM140749NIGMS NIH HHS RM1 GM149374
6 · The paper itself

Abstract

We illustrate the utility of native mass spectrometry (nMS) combined with a fast, tunable gas-phase charge reduction, electron capture charge reduction (ECCR), for the characterization of protein complex topology and glycoprotein heterogeneity. ECCR efficiently reduces the charge states of tetradecameric GroEL, illustrating Orbitrap

Identifiers

PMID38496594
PMCPMC10942452
OpenAlexW4392633756

What OpenQuestion holds

Textmetadata
LicenceCC BY-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.