Evidence map›Paper›PMID 38495715›Full record

ArticleBiomedical optics express2024

Dynamic speckle illumination wide-field fluorescence microscopy with actively optical manipulation of rotational angles.

Jun Yin, Yan Miao, Ruijing Liang, Haoyi Hou, Chuanxin Teng, Lingyao Yu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Jun YinPhotonics Research Center, School of Optoelectronic Engineering, Guilin University of Electronic Technology, Guilin 541004, China.
Yan MiaoPhotonics Research Center, School of Optoelectronic Engineering, Guilin University of Electronic Technology, Guilin 541004, China.
Ruijing LiangPhotonics Research Center, School of Optoelectronic Engineering, Guilin University of Electronic Technology, Guilin 541004, China.
Haoyi HouPhotonics Research Center, School of Optoelectronic Engineering, Guilin University of Electronic Technology, Guilin 541004, China.
Chuanxin TengPhotonics Research Center, School of Optoelectronic Engineering, Guilin University of Electronic Technology, Guilin 541004, China.
Lingyao YuPhotonics Research Center, School of Optoelectronic Engineering, Guilin University of Electronic Technology, Guilin 541004, China.
Guilin University of Electronic Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We present a dynamic speckle illumination wide-field fluorescence microscopy (DSIWFM) combined with a line optical tweezers (LOTs) for rotational fluorescence sectioning imaging. In this method, large polystyrene fluorescent microspheres are stably trapped with LOTs, and precisely manipulated to rotate around a specific rotation axis. During the rotation process, multiple raw fluorescence images of trapped microspheres are obtained with dynamic speckle illumination. The root-mean-square (RMS) algorithm is used to extract the drastically changing fluorescent signals in the focal plane to obtain the fluorescence sectioning images of the samples at various angles. The influence of speckle granularity on the image quality of fluorescence sectioning images is experimentally analyzed. The rotational fluorescence sectioning images obtained by DSIWFM with LOTs could provide an alternative technique for applications of biomedical imaging.

Identifiers

PMID38495715
PMCPMC10942717
OpenAlexW4391436360

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.