Evidence map›Paper›PMID 38495683›Full record

ArticleBiomedical optics express2024

Quantifying nanoscopic alterations associated with mitochondrial dysfunction using three-dimensional single-molecule localization microscopy.

Benjamin Brenner, Fengyuanshan Xu, Yang Zhang, Junghun Kweon, Raymond Fang, Nader Sheibani, Sarah X Zhang, Cheng Sun, Hao F Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Benjamin BrennerDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, USA.ORCID https://orcid.org/0000-0003-4736-1789
Fengyuanshan XuDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, USA.
Yang ZhangDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, USA.
Junghun KweonDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, USA.
Raymond FangDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, USA.ORCID https://orcid.org/0000-0002-0647-8537
Nader SheibaniDepartment of Ophthalmology and Vision Sciences, University of Wisconsin, Madison, WI, USA.
Sarah X ZhangDepartment of Ophthalmology, University at Buffalo, Buffalo, NY, USA.
Cheng SunDepartment of Mechanical Engineering, Northwestern University, Evanston, IL, USA.
Hao F ZhangDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, USA.ORCID https://orcid.org/0000-0001-5089-1196
Northwestern University · USUniversity at Buffalo, State University of New York · US

Funding

ER Stress and Diabetic RetinopathyR01EY019949 · NEI · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI ZHANG, SARAH X · 2010 to 2023
$5.5M
Molecular Mechanisms of Severe Diabetic RetinopathyR01EY030970 · NEI · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI Sarah X Zhang · 2020 to 2026
$3.1M
Physical Genomics and Engineering Training ProgramT32GM142604 · NIGMS · NORTHWESTERN UNIVERSITY · PI BACKMAN, VADIM · 2021 to 2025
$1.9M
Photoactivatable Fluorophores for High-Throughput Multiplexed Tracking of Single-Molecules in Live CellsR01GM143397 · NIGMS · UNIVERSITY OF MIAMI CORAL GABLES · PI RAYMO, FRANCISCO M · 2022 to 2025
$1.4M
NEI NIH HHS R01 EY019949NEI NIH HHS R01 EY030970NIGMS NIH HHS R01 GM143397NIGMS NIH HHS T32 GM142604
6 · The paper itself

Abstract

Mitochondrial morphology provides unique insights into their integrity and function. Among fluorescence microscopy techniques, 3D super-resolution microscopy uniquely enables the analysis of mitochondrial morphological features individually. However, there is a lack of tools to extract morphological parameters from super-resolution images of mitochondria. We report a quantitative method to extract mitochondrial morphological metrics, including volume, aspect ratio, and local protein density, from 3D single-molecule localization microscopy images, with single-mitochondrion sensitivity. We validated our approach using simulated ground-truth SMLM images of mitochondria. We further tested our morphological analysis on mitochondria that have been altered functionally and morphologically in controlled manners. This work sets the stage to quantitatively analyze mitochondrial morphological alterations associated with disease progression on an individual basis.

Identifiers

PMID38495683
PMCPMC10942681
OpenAlexW4391574340

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.