ArticleMicrobiome2024
Global abundance patterns, diversity, and ecology of Patescibacteria in wastewater treatment plants.
Article in Microbiome, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Micro-electric field-associated restructuring of bacterial communities enhances wastewater purification in constructed wetlands.iScience · 2026Article
- TreeProfiler: large-scale metadata profiling along gene and species trees.Molecular biology and evolution · 2026Article
- Correction: Global abundance patterns, diversity, and ecology of Patescibacteria in wastewater treatment plants.Microbiome · 2026Article
- Revised 16S rRNA V4 hypervariable region targeting primers enhance detection of Patescibacteria and other lineages across diverse environments.ISME communications · 2026Article
- Current Understanding of Taxonomy and Ecology of the Phylum Minisyncoccota.Microbes and environments · 2026Review
- Metabolic Potential of Candidatus Saccharimonadia Including Rare Lineages in Activated Sludge.Environmental microbiology reports · 2025Article
- National-scale biogeography and function of river and stream bacterial biofilm communities.Nature communications · 2025Article
- Microbial diversity supports nitrification: insights from a full-scale anoxic/oxic wastewater treatment process.Applied and environmental microbiology · 2025Article
- Diverse Patescibacteria assemblages and prevalence of ultra-small free-living Parcubacteria along a subterranean estuary.mSystems · 2025Article
- Growth of candidate phyla radiation bacteria in groundwater incubations reveals widespread adaptations to oxic conditions.Microbiome · 2025Article
- Seasonal dynamics of microbial diversity and functional potential in active sanitary landfill baseliner microbiomes.Biodegradation · 2025Article
- The effect of maternal sodium ρ-perfluorous nonenoxybenzene sulfonate exposure on the gut microbiota in dams and offspring.Scientific reports · 2025Article
- Structural and Functional Differences in the Gut and Lung Microbiota of Pregnant Pomona Leaf-Nosed Bats.Microorganisms · 2025Article
- Two novel Patescibacteria:mBio · 2025Article
- CrAssphage distribution analysis in an Amazonian river based on metagenomic sequencing data and georeferencing.Applied and environmental microbiology · 2025Article
- Multi-Metagenome Analysis Unravels Community Collapse After Sampling and Hints the Cultivation Strategy of CPR Bacteria in Groundwater.Microorganisms · 2025Article
- Candidate Phyla Radiation (CPR) bacteria from hyperalkaline ecosystems provide novel insight into their symbiotic lifestyle and ecological implications.Microbiome · 2025Article
- Mutations in Gordonia amarae mycolic acid biosynthetic pathway confer resistance to Patescibacteria parasite Mycosynbacter amalyticus.Nature communications · 2025Article
- Coupled N and P cycling as driven by microbial taxa and interactions.Frontiers in microbiology · 2025Article
- Nitrifying Communities in Biological Nitrogen Removal Processes at Tropical Municipal Wastewater Treatment Plants.Microbes and environments · 2025Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors.
Funding
Abstract
backgroundMicroorganisms are responsible for nutrient removal and resource recovery in wastewater treatment plants (WWTPs), and their diversity is often studied by 16S rRNA gene amplicon sequencing. However, this approach underestimates the abundance and diversity of Patescibacteria due to the low coverage of commonly used PCR primers for this highly divergent bacterial phylum. Therefore, our current understanding of the global diversity, distribution, and ecological role of Patescibacteria in WWTPs is very incomplete. This is particularly relevant as Patescibacteria are considered to be associated with microbial host cells and can therefore influence the abundance and temporal variability of other microbial groups that are important for WWTP functioning.
resultsHere, we evaluated the in silico coverage of widely used 16S rRNA gene-targeted primer pairs and redesigned a primer pair targeting the V4 region of bacterial and archaeal 16S rRNA genes to expand its coverage for Patescibacteria. We then experimentally evaluated and compared the performance of the original and modified V4-targeted primers on 565 WWTP samples from the MiDAS global sample collection. Using the modified primer pair, the percentage of ASVs classified as Patescibacteria increased from 5.9 to 23.8%, and the number of detected patescibacterial genera increased from 560 to 1576, while the detected diversity of the remaining microbial community remained similar. Due to this significantly improved coverage of Patescibacteria, we identified 23 core genera of Patescibacteria in WWTPs and described the global distribution pattern of these unusual microbes in these systems. Finally, correlation network analysis revealed potential host organisms that might be associated with Patescibacteria in WWTPs. Interestingly, strong indications were found for an association between Patescibacteria of the Saccharimonadia and globally abundant polyphosphate-accumulating organisms of the genus Ca. Phosphoribacter.
conclusionsOur study (i) provides an improved 16S rRNA gene V4 region-targeted amplicon primer pair inclusive of Patescibacteria with little impact on the detection of other taxa, (ii) reveals the diversity and distribution patterns of Patescibacteria in WWTPs on a global scale, and (iii) provides new insights into the ecological role and potential hosts of Patescibacteria in WWTPs. Video Abstract.
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