Evidence map›Paper›PMID 38491258›Full record

ArticleBulletin of experimental biology and medicine2024

Evaluation of Collagenase Activity from Crab Hepatopancreas in Different Model Systems.

N G Plekhova, I N Chernenko, D V Korolev, E P Kozlovskaya, A K Stepanyugina, O V Shevchenko, P S Dmitrenok, V B Shumatov

Abstract read
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In one paragraph

Article in Bulletin of experimental biology and medicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

N G PlekhovaPacific State Medical University, Ministry of Health of the Russian Federation, Vladivostok, Russia. pl_nat@hotmail.com.
I N ChernenkoPacific State Medical University, Ministry of Health of the Russian Federation, Vladivostok, Russia.
D V KorolevPacific State Medical University, Ministry of Health of the Russian Federation, Vladivostok, Russia.
E P KozlovskayaG. B. Elyakov Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Vladivostok, Russia.
A K StepanyuginaPacific State Medical University, Ministry of Health of the Russian Federation, Vladivostok, Russia.
O V ShevchenkoPacific State Medical University, Ministry of Health of the Russian Federation, Vladivostok, Russia.
P S DmitrenokG. B. Elyakov Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Vladivostok, Russia.
V B ShumatovPacific State Medical University, Ministry of Health of the Russian Federation, Vladivostok, Russia.
Vladivostok State Medical University · RUPacific Institute of Bioorganic Chemistry. GB Elyakova Far Eastern Branch of the Russian Academy of Sciences · RUMinistry of Health of the Russian Federation · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The effect of Kamchatka crab hepatopancreas containing three collagenolytic isoenzymes Collagenase KK and proteinases of Streptomyces lavendulae on metabolic activity and cell death were carried out on in vitro models. It was shown that changes in the protein structure under the influence of Collagenase KK occurred earlier than under the effect of bacterial proteinases. At the same time, activity of Collagenase KK was significantly higher than that of bacterial proteinases (p<0.01). Both preparations had a pronounced time- and dose-dependent effects on metabolic activity of cells. Collagenase KK had low cytotoxic effect, and cells mainly died by apoptosis. Thus, hepatopancreas collagenase has a high activity and proapoptotic effect on cells and can be used in low concentrations for enzymatic disaggregation of tissues.

Indexed as

BrachyuraAnimalsCollagenasesEndopeptidasesHepatopancreasPeptide HydrolasesCollagenasesEndopeptidasesPeptide Hydrolasesbiopsiescell technologiescollagenaseenzymatic disaggregation

Identifiers

PMID38491258
OpenAlexW4392857749

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.