Evidence map›Paper›PMID 38491149›Full record

ArticleAnalytical and bioanalytical chemistry2024

Development of alkaline phosphatase-linked single-chain variable fragment fusion proteins for one-step immunodetection of deoxynivalenol in cereals.

Li Wen, Yirui Huang, Zhichang Sun, Shiyuan Shi, Xiaoxia Xie, Zhenyun He, Xing Liu

Abstract read
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In one paragraph

Article in Analytical and bioanalytical chemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.4field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Li WenSchool of Food Science and Engineering, Hainan University, Haikou, 570228, China.
Yirui HuangSchool of Food Science and Engineering, Hainan University, Haikou, 570228, China.
Zhichang SunSchool of Food Science and Engineering, Hainan University, Haikou, 570228, China.
Shiyuan ShiSchool of Food Science and Engineering, Hainan University, Haikou, 570228, China.
Xiaoxia XieSchool of Food Science and Engineering, Hainan University, Haikou, 570228, China.
Zhenyun HeSchool of Food Science and Engineering, Hainan University, Haikou, 570228, China. zhenyun89@foxmail.com.
Xing LiuSchool of Food Science and Engineering, Hainan University, Haikou, 570228, China. xliu@hainanu.edu.cn.ORCID http://orcid.org/0000-0002-1301-4837
Hainan University · CN

Funding

Education Department of Hainan Province Hnky2023-74National Natural Science Foundation of China 32102067Natural Science Foundation of Hainan Province 323QN251Natural Science Foundation of Hainan Province 824MS074
6 · The paper itself

Abstract

Deoxynivalenol (DON) is a mycotoxin that widely distributes in various foods and seriously threatens food safety. To minimize the consumers' dietary exposure to DON, there is an urgent demand for developing rapid and sensitive detection methods for DON in food. In this study, a bifunctional single-chain variable fragment (scFv) linked alkaline phosphatase (ALP) fusion protein was developed for rapid and sensitive detection of deoxynivalenol (DON). The scFv gene was chemically synthesized and cloned into the expression vector pET25b containing the ALP gene by homologous recombination. The prokaryotic expression, purification, and activity analysis of fusion proteins (scFv-ALP and ALP-scFv) were well characterized and performed. The interactions between scFv and DON were investigated by computer-assisted simulation, which included hydrogen bonds, hydrophobic interactions, and van der Waals forces. The scFv-ALP which showed better bifunctional activity was selected for developing a direct competitive enzyme-linked immunosorbent assay (dc-ELISA) for DON in cereals. The dc-ELISA takes 90 min for one test and exhibits a half inhibitory concentration (IC

Indexed as

Alkaline PhosphataseEdible GrainEnzyme-Linked Immunosorbent AssayRecombinant Fusion ProteinsSingle-Chain AntibodiesTrichothecenesFood ContaminationLimit of DetectionAlkaline PhosphatasedeoxynivalenolRecombinant Fusion ProteinsSingle-Chain AntibodiesTrichothecenesAlkaline phosphataseCerealImmunoassayMycotoxinSingle-chain variable fragment

Identifiers

PMID38491149
OpenAlexW4392884533

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.