ArticleeLife2024
The molecular mechanism and evolutionary divergence of caspase 3/7-regulated gasdermin E activation.
Article in eLife, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Theme and variations: activation and regulation of gasdermin-mediated inflammation.Biochemical Society transactions · 2026Review
- Structural basis and regulation of GSDME pore formation.Nature communications · 2026Article
- Harnessing pyroptosis in breast cancer therapy: immunological mechanisms and emerging biomaterial strategies.Cell death discovery · 2026Review
- Gasdermins against intracellular bacterial pathogens.Nature microbiology · 2026Review
- Caspase-mediated interleukin-18 maturation promotes membrane-associated unconventional secretion and signal transduction in a teleost fish.Marine life science & technology · 2026Article
- Teleost GSDMEc regulates GSDMEa-mediated pyroptosis.Journal of advanced research · 2026Article
- Insights into the olaparib-mediated cell death mechanisms in canine hematological malignancies: a different fate for CLBL-1 and GL-1 cell lines.Frontiers in veterinary science · 2026Article
- Apoptosis as an evolutionary battleground: pathogen pressure and the shaping of programmed cell death pathways.Frontiers in cell and developmental biology · 2026Review
- Ninjurin-1 executes plasma membrane rupture and impairs anti-microbial immunity in an early jawed vertebrate.Cellular and molecular life sciences : CMLS · 2025Article
- Antiproliferative and apoptotic effects of Pervari honey on SH-SY5Y neuroblastoma cells.Medical oncology (Northwood, London, England) · 2025Article
- Caspases: structural and molecular mechanisms and functions in cell death, innate immunity, and disease.Cell discovery · 2025Review
- Caspase-7 and Vitamin D Receptor Gene as Key Genes of Hypertension Caused by Pyroptosis in Human.Journal of clinical hypertension (Greenwich, Conn.) · 2025Article
- Long non-coding RNA AC133552.2: biomarker and therapeutic target in osteosarcoma via PANoptosis gene screening.Scientific reports · 2025Article
- Pufferfish gasdermin Ea is a significant player in the defense against bacterial pathogens.Marine life science & technology · 2024Article
- Characterization of the Apoptotic and Antimicrobial Activities of Two Initiator Caspases of Sea CucumberGenes · 2024Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
Abstract
Caspase (CASP) is a family of proteases involved in cleavage and activation of gasdermin, the executor of pyroptosis. In humans, CASP3 and CASP7 recognize the same consensus motif DxxD, which is present in gasdermin E (GSDME). However, human GSDME is cleaved by CASP3 but not by CASP7. The underlying mechanism of this observation is unclear. In this study, we identified a pyroptotic pufferfish GSDME that was cleaved by both pufferfish CASP3/7 and human CASP3/7. Domain swapping between pufferfish and human CASP and GSDME showed that the GSDME C-terminus and the CASP7 p10 subunit determined the cleavability of GSDME by CASP7. p10 contains a key residue that governs CASP7 substrate discrimination. This key residue is highly conserved in vertebrate CASP3 and in most vertebrate (except mammalian) CASP7. In mammals, the key residue is conserved in non-primates (e.g., mouse) but not in primates. However, mouse CASP7 cleaved human GSDME but not mouse GSDME. These findings revealed the molecular mechanism of CASP7 substrate discrimination and the divergence of CASP3/7-mediated GSDME activation in vertebrate. These results also suggested that mutation-mediated functional alteration of CASP probably enabled the divergence and specialization of different CASP members in the regulation of complex cellular activities in mammals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.