ArticleBiological imaging2023
FLUTE: A Python GUI for interactive phasor analysis of FLIM data.
Article in Biological imaging, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- FLIMExplorer: interactive GUI for object-based visualization and analysis of fluorescence lifetime images.JPhys photonics · 2026Article
- Emerging trends of fluorescence lifetime imaging microscopy (FLIM): advances, challenges, and prospects.Biophysics reports · 2026Article
- FLIM Playground: An interactive, end-to-end graphical user interface for analyzing single cells with fluorescence lifetime imaging microscopy.Cell reports methods · 2026Article
- Automated FLIM-FRET Segmentation Within RNP Condensates.Bio-protocol · 2026Article
- Chemical fluorophores for fluorescence lifetime imaging.Chemical Society reviews · 2026Review
- A fluorescence lifetime separation approach for FLIM live-cell imaging.Journal of microscopy · 2026Article
- Engineering macrophage responses through 3D scaffold microarchitecture.Materials today. Bio · 2025Article
- Label-free nonlinear microscopy probes cellular metabolism and myelin dynamics in live tissue.Communications biology · 2025Article
- FLIM Playground: An interactive, end-to-end graphical user interface for analyzing single cells with fluorescence lifetime imaging microscopy.bioRxiv : the preprint server for biology · 2025Article
- Phasor theory of fluorescence lifetime imaging utilized on a maximum range of frequencies for prostate tissue analysis.Biomedical optics express · 2025Article
- FLIMPA: A Versatile Software for Fluorescence Lifetime Imaging Microscopy Phasor Analysis.Analytical chemistry · 2025Article
- FLIMB: fluorescence lifetime microendoscopy for metabolic and functional imaging of femoral marrow at subcellular resolution.Biomedical optics express · 2025Article
- Calibration approaches for fluorescence lifetime applications using time-domain measurements.Methods in microscopy · 2025Article
- GSLab: open-source platform for advanced phasor analysis in fluorescence microscopy.Bioinformatics (Oxford, England) · 2025Article
- GSLab: Open-Source Platform for Advanced Phasor Analysis in Fluorescence Microscopy.bioRxiv : the preprint server for biology · 2025Article
- Consensus guidelines for cellular label-free optical metabolic imaging: ensuring accuracy and reproducibility in metabolic profiling.Journal of biomedical optics · 2025Article
- Fluorescence lifetime sorting reveals tunable enzyme interactions within cytoplasmic condensates.The Journal of cell biology · 2025Article
- Pioglitazone Phases and Metabolic Effects in Nanoparticle-Treated Cells Analyzed via Rapid Visualization of FLIM Images.Molecules (Basel, Switzerland) · 2024Article
- Investigating the relationship between hypoxia, hypoxia-inducible factor 1, and the optical redox ratio in response to radiation therapy.Biophotonics discovery · 2024Article
- Phasor identifier: A cloud-based analysis of phasor-FLIM data on Python notebooks.Biophysical reports · 2023Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique used to probe the local environment of fluorophores. The fit-free phasor approach to FLIM data is increasingly being used due to its ease of interpretation. To date, no open-source graphical user interface (GUI) for phasor analysis of FLIM data is available in Python, thus limiting the widespread use of phasor analysis in biomedical research. Here, we present Fluorescence Lifetime Ultimate Explorer (FLUTE), a Python GUI that is designed to fill this gap. FLUTE simplifies and automates many aspects of the analysis of FLIM data acquired in the time domain, such as calibrating the FLIM data, performing interactive exploration of the phasor plot, displaying phasor plots and FLIM images with different lifetime contrasts simultaneously, and calculating the distance from known molecular species. After applying desired filters and thresholds, the final edited datasets can be exported for further user-specific analysis. FLUTE has been tested using several FLIM datasets including autofluorescence of zebrafish embryos and in vitro cells. In summary, our user-friendly GUI extends the advantages of phasor plotting by making the data visualization and analysis easy and interactive, allows for analysis of large FLIM datasets, and accelerates FLIM analysis for non-specialized labs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.