Evidence map›Paper›PMID 38486857›Full record

ArticleTechnology

Macrophage modulation by polymerized hemoglobins: Potential as a wound-healing therapy.

Paulina Krzyszczyk, Kishan Patel, Yixin Meng, Maurice O'Reggio, Kristopher Richardson, Alison Acevedo, Ioannis P Androulakis, Martin L Yarmush, Rene S Schloss, Andre F Palmer and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Technology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 77% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Paulina KrzyszczykDepartment of Biomedical Engineering, Rutgers University, Piscataway, NJ 08854, USA.
Kishan PatelDepartment of Biomedical Engineering, Rutgers University, Piscataway, NJ 08854, USA.
Yixin MengDepartment of Biomedical Engineering, Rutgers University, Piscataway, NJ 08854, USA.
Maurice O'ReggioDepartment of Biomedical Engineering, Rutgers University, Piscataway, NJ 08854, USA.
Kristopher RichardsonDepartment of Chemical & Biomolecular Engineering, The Ohio State University, Columbus, OH 43210, USA.
Alison AcevedoDepartment of Biomedical Engineering, Rutgers University, Piscataway, NJ 08854, USA.
Ioannis P AndroulakisDepartment of Biomedical Engineering, Rutgers University, Piscataway, NJ 08854, USA.
Martin L YarmushDepartment of Biomedical Engineering, Rutgers University, Piscataway, NJ 08854, USA.
Rene S SchlossDepartment of Biomedical Engineering, Rutgers University, Piscataway, NJ 08854, USA.
Andre F PalmerDepartment of Chemical & Biomolecular Engineering, The Ohio State University, Columbus, OH 43210, USA.
Franҫois BerthiaumeDepartment of Biomedical Engineering, Rutgers University, Piscataway, NJ 08854, USA.
Rutgers, The State University of New Jersey · USThe Ohio State University · US

Funding

UMDNJ TRAINING GRANT IN BIOTECHNOLOGYT32GM008339 · NIGMS · RUTGERS THE ST UNIV OF NJ NEW BRUNSWICK · PI YARMUSH, MARTIN L · 1989 to 2019
$6.1M
PEGylated megahemoglobin for use as a red blood cell substituteR01HL138116 · NHLBI · OHIO STATE UNIVERSITY · PI CABRALES, PEDRO, KAUMAYA, PRAVIN T. P. · 2017 to 2020
$2.9M
Attenuating the Oxidative and Myocardial Side-Effects of Acellular HemoglobinR01HL126945 · NHLBI · OHIO STATE UNIVERSITY · PI CABRALES, PEDRO, PALMER, ANDRE FRANCIS · 2016 to 2019
$1.7M
Polymerized Hemoglobins for Facilitated Oxygen Transport in Hepatic BioreactorsR01EB021926 · NIBIB · OHIO STATE UNIVERSITY · PI BERTHIAUME, FRANCOIS, PALMER, ANDRE FRANCIS · 2016 to 2019
$1.4M
Attenuating the Oxidative and Myocardial Toxicity of Polymerized HemoglobinsR56HL123015 · NHLBI · OHIO STATE UNIVERSITY · PI CABRALES, PEDRO, PALMER, ANDRE FRANCIS · 2014 to 2014
$400k
NHLBI NIH HHS R01 HL126945NHLBI NIH HHS R01 HL138116NHLBI NIH HHS R56 HL123015NIBIB NIH HHS R01 EB021926NIGMS NIH HHS T32 GM008339
6 · The paper itself

Abstract

Chronic skin wounds are hypoxic and are stalled in a pro-inflammatory state. Hemoglobin (Hb)-based oxygen carriers have shown potential in increasing oxygen delivery to aid wound healing. Macrophages also take up Hb, thus altering their phenotype and the regulation of inflammation. Herein, we compared the effect of Hb and polymerized Hbs (PolyHbs) on the phenotype of human macrophages. Macrophages were incubated with Hb or different forms of PolyHbs, and the inflammatory secretion profile was analyzed. PolyHbs were produced by polymerizing Hb in the relaxed (R) or tense (T) quaternary state and by varying the molar ratio of the glutaraldehyde crosslinking agent to Hb. Hb decreased the secretion of most measured factors. PolyHb treatment led to generally similar secretion profiles; however, Hb had more similar trends to R-state PolyHb. Ingenuity pathway analysis predicted positive outcomes in wound healing and angiogenesis for T-state PolyHb prepared with a 30:1 (glutaraldehyde:Hb) polymerization ratio. When tested in diabetic mouse wounds, T-state PolyHb resulted in the greatest epidermal thickness and vascular endothelial CD31 staining. Thus, the effects of PolyHb on macrophages are affected by the polymerization ratio and the quaternary state, and T-state PolyHb yields secretion profiles that are most beneficial in wound healing.

Indexed as

Chronic Wound HealingInflammationMacrophagesPolymerized Hemoglobin

Identifiers

PMID38486857
PMCPMC10938467
OpenAlexW3001227362

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.