ArticleBMC infectious diseases2024
Isolation and characterization of lytic bacteriophages from various sources in Addis Ababa against antimicrobial-resistant diarrheagenic Escherichia coli strains and evaluation of their therapeutic potential.
Article in BMC infectious diseases, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 28 citations in OpenAlex.
- Article
- Antibiofilm efficacy and genomic characterization of a lytic bacteriophage targeting MDR uropathogenic Escherichia coli.Archives of microbiology · 2026Article
- Comparative Genomic and Functional Characterization of Two Lytic Bacteriophages Against Antimicrobial-ResistantAntibiotics (Basel, Switzerland) · 2026Article
- Characterization and evaluation of a phage cocktail targeting Salmonella enterica in a Turkey farm.BMC microbiology · 2026Article
- Isolation and characterization of lytic bacteriophages with therapeutic potential against multidrug resistant Klebsiella pneumoniae from Ethiopia.Scientific reports · 2026Article
- Phage cocktails: state-of-the-art technologies and strategies for effective design.FEMS microbiology reviews · 2026Review
- Characterization and phage-antibiotic synergy of bacteriophages PES-1 and PES-2 against porcine enterotoxigenicFrontiers in microbiology · 2026Article
- Efficacy ofFrontiers in microbiology · 2026Article
- Isolation of Bacteriophages and Their Lytic Profile From River and Waste Water Samples in South Western Uganda.Cureus · 2026Article
- Observational
- Draft genome sequence and intact prophage characterisation of multidrug-resistantData in brief · 2025Article
- Pathogen-Phage Geomapping to Overcome Resistance.bioRxiv : the preprint server for biology · 2025Article
- Emergence of extensively and pan-drug resistance in clinical bacterial isolates: A systematic scoping review from Ethiopian public health perspective.PLoS neglected tropical diseases · 2025Article
- Isolation and Genomic Analysis ofAntibiotics (Basel, Switzerland) · 2025Article
- Detection of Antibiotic-Resistance Genes in Drinking Water: A Study at a University in the Peruvian Amazon.International journal of environmental research and public health · 2025Article
- Bacteriophages: A Challenge for Antimicrobial Therapy.Microorganisms · 2025Review
- Article
- Characterization and Comparative Genomic Analysis of vB_BceM_CEP1: A Novel Temperate Bacteriophage Infecting Burkholderia cepacia Complex.Journal of microbiology (Seoul, Korea) · 2024Article
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2 authors at 1 institution in 1 country.
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Abstract
backgroundEscherichia coli is a common fecal coliform, facultative aerobic, gram-negative bacterium. Pathogenic strains of such microbes have evolved to cause diarrhea, urinary tract infections, and septicemias. The emergence of antibiotic resistance urged the identification of an alternative strategy. The use of lytic bacteriophages against the control of pathogenic E. coli in clinics and different environmental setups (waste and drink water management) has become an alternative therapy to antibiotic therapy. Thus, this study aimed to isolate and characterize lytic bacteriophage from various sources in Addis Ababa, tested them against antimicrobial-resistant diarrheagenic E. coli strains and evaluated their therapeutic potential under in vitro conditions.
methodsA total of 14 samples were processed against six different diarrheagenic E. coli strains. The conventional culture and plaque analysis agar overlay method was used to recover lytic bacteriophage isolates. The phage isolates were characterized to determine their lytic effect, growth characteristics, host range activity, and stability under different temperature and pH conditions. Phage isolates were identified by scanning electron microscope (SEM), and molecular techniques (PCR).
resultsIn total, 17 phages were recovered from 84 tested plates. Of the 17 phage isolates, 11 (65%) were Myoviridae-like phages, and 6 (35%) phage isolates were Podoviridae and Siphoviridae by morphology and PCR identification. Based on the host range test, growth characteristics, and stability test 7 potent phages were selected. These phages demonstrated better growth characteristics, including short latent periods, highest burst sizes, and wider host ranges, as well as thermal stability and the ability to survive in a wide range of pH levels.
conclusionsThe promising effect of the phages isolated in this study against AMR pathogenic E. coli has raised the possibility of their use in the future treatment of E. coli infections.
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