Evidence map›Paper›PMID 38483908›Full record

ArticleCell reports2024

Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution.

Michelle K Tenggara, Seo-Ho Oh, Catherine Yang, Hardik K Nariya, Amanda M Metz, Amit A Upadhyay, Dedeepya R Gudipati, Lizheng Guo, Emily G McGhee, Kiran Gill and 12 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors.

Michelle K TenggaraEmory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA.
Seo-Ho OhEmory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA.
Catherine YangEmory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA.
Hardik K NariyaEmory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA.
Amanda M MetzEmory National Primate Research Center, Atlanta, GA 30329, USA.
Amit A UpadhyayEmory National Primate Research Center, Atlanta, GA 30329, USA.
Dedeepya R GudipatiEmory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA.
Lizheng GuoEmory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA.
Emily G McGheeEmory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA.
Kiran GillEmory National Primate Research Center, Atlanta, GA 30329, USA.
Elise G VioxVaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA.
Rosemarie D MasonVaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA.
Nicole A Doria-RoseVaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA.
Kathryn E FouldsVaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA.
John R MascolaVaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA.
Yuhong DuDepartment of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, GA 30322, USA; Emory Chemical Biology Discovery Center, Emory University School of Medicine, Atlanta, GA 30322, USA.
Haian FuDepartment of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, GA 30322, USA; Emory Chemical Biology Discovery Center, Emory University School of Medicine, Atlanta, GA 30322, USA; Department of Hematology and Medical Oncology and Winship Cancer Institute, Emory University, Atlanta, GA 30322, USA.
John D AltmanEmory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA; Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322, USA.
Qi YanDepartment of Obstetrics and Gynecology, Columbia University Irving Medical Center, New York, NY 10032, USA.
Zizhang ShengAaron Diamond AIDS Research Center, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032, USA.
Steven E BosingerEmory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA; Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA.
Rui KongEmory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA; Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA. Electronic address: rui.kong@emory.edu.

Funding

Yerkes National Primate Research Center Role of type-I IFN in regulating COVID-19 induced inflammation and pathogenesisP51OD011132 · OD · EMORY UNIVERSITY · PI Joon Sup Lee · 2012 to 2026
$167.0M
Virology and Molecular Biomarkers CoreP30AI050409 · NIAID · EMORY UNIVERSITY · PI Ann M Chahroudi, Colleen F Kelley · 2002 to 2026
$74.0M
Simultaneous antigen receptor repertoire profiling and single-cell transcriptomics in T and B lymphocytes from limited clinical samplesU24AI120134 · NIAID · EMORY UNIVERSITY · PI BOSINGER, STEVEN EDWARD · 2015 to 2024
$6.5M
HIV-1 Fusion Peptide-directed Vaccine Design Using Virus-like ParticlesR01AI162267 · NIAID · EMORY UNIVERSITY · PI Rui Kong · 2021 to 2026
$5.7M
Quick and Accurate Measurements of HIV Broadly Neutralizing Antibody SusceptibilityR61AI176583 · NIAID · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI SHENG, ZIZHANG, WU, XUELING · 2023 to 2025
$3.0M
Illumina NovaSeq 6000 High Throughput DNA Sequencer for Emory UniversityS10OD026799 · OD · EMORY UNIVERSITY · PI BOSINGER, STEVEN EDWARD · 2019 to 2019
$985k
NIAID NIH HHS P30 AI050409NIAID NIH HHS R01 AI162267NIAID NIH HHS R61 AI176583NIAID NIH HHS U24 AI120134NIH HHS P51 OD011132NIH HHS S10 OD026799
6 · The paper itself

Abstract

Identifying individual functional B cell receptors (BCRs) is common, but two-dimensional analysis of B cell frequency versus BCR potency would delineate both quantity and quality of antigen-specific memory B cells. We efficiently determine quantitative BCR neutralizing activities using a single-cell-derived antibody supernatant analysis (SCAN) workflow and develop a frequency-potency algorithm to estimate B cell frequencies at various neutralizing activity or binding affinity cutoffs. In an HIV-1 fusion peptide (FP) immunization study, frequency-potency curves elucidate the quantity and quality of FP-specific immunoglobulin G (IgG)+ memory B cells for different animals, time points, and antibody lineages at single-cell resolution. The BCR neutralizing activities are mainly determined by their affinities to soluble envelope trimer. Frequency analysis definitively demonstrates dominant neutralizing antibody lineages. These findings establish SCAN and frequency-potency analyses as promising approaches for general B cell analysis and monoclonal antibody (mAb) discovery. They also provide specific rationales for HIV-1 FP-directed vaccine optimization.

Indexed as

HIV-1HIV InfectionsHIV SeropositivityAnimalsAntibodies, NeutralizingHIV AntibodiesImmunoglobulin GMemory B CellsAntibodies, NeutralizingHIV AntibodiesImmunoglobulin GantibodyB cell receptorBCRCP: Immunologyfrequencyfusion peptideHIV-1potencySCANsingle-cell-derived antibody supernatant analysisvaccine

Identifiers

PMID38483908
PMCPMC11003769

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.