Evidence map›Paper›PMID 38481131›Full record

ArticleClinical proteomics2024

Human tear film protein sampling using soft contact lenses.

Robert K Roden, Nathan Zuniga, Joshua C Wright, David H Parkinson, Fangfang Jiang, Leena M Patil, Rebecca S Burlett, Alyssa A Nitz, Joshua J Rogers, Jarett T Pittman and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical proteomics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Ocular tear fluid biomarkers collected by contact lenses.Biochemical and biophysical research communications · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Robert K RodenDepartment of Chemistry & Biochemistry, Brigham Young University, Provo, UT, 84602, USA.
Nathan ZunigaDepartment of Chemistry & Biochemistry, Brigham Young University, Provo, UT, 84602, USA.
Joshua C WrightDepartment of Chemistry & Biochemistry, Brigham Young University, Provo, UT, 84602, USA.
David H ParkinsonDepartment of Chemistry & Biochemistry, Brigham Young University, Provo, UT, 84602, USA.
Fangfang JiangDepartment of Chemistry & Biochemistry, Brigham Young University, Provo, UT, 84602, USA.
Leena M PatilDepartment of Chemistry & Biochemistry, Brigham Young University, Provo, UT, 84602, USA.
Rebecca S BurlettDepartment of Chemistry & Biochemistry, Brigham Young University, Provo, UT, 84602, USA.
Alyssa A NitzDepartment of Biology, Brigham Young University, Provo, UT, 84602, USA.
Joshua J RogersDepartment of Chemistry & Biochemistry, Brigham Young University, Provo, UT, 84602, USA.
Jarett T PittmanDepartment of Chemistry & Biochemistry, Brigham Young University, Provo, UT, 84602, USA.
Kenneth L VirginDepartment of Chemistry & Biochemistry, Brigham Young University, Provo, UT, 84602, USA.
P Christine AckroydDepartment of Chemistry & Biochemistry, Brigham Young University, Provo, UT, 84602, USA.
Samuel H PayneDepartment of Biology, Brigham Young University, Provo, UT, 84602, USA.
John C PriceDepartment of Chemistry & Biochemistry, Brigham Young University, Provo, UT, 84602, USA.
Kenneth A ChristensenDepartment of Chemistry & Biochemistry, Brigham Young University, Provo, UT, 84602, USA. ken.christensen@byu.edu.

Funding

Biochemical Consequences of Regiospecific Metabolic Bias in the BrainR01AG066874 · NIA · BRIGHAM YOUNG UNIVERSITY · PI PRICE, JOHN C. · 2020 to 2024
$3.0M
NIA NIH HHS R01 AG066874
6 · The paper itself

Abstract

backgroundHuman tear protein biomarkers are useful for detecting ocular and systemic diseases. Unfortunately, existing tear film sampling methods (Schirmer strip; SS and microcapillary tube; MCT) have significant drawbacks, such as pain, risk of injury, sampling difficulty, and proteomic disparities between methods. Here, we present an alternative tear protein sampling method using soft contact lenses (SCLs).

resultsWe optimized the SCL protein sampling in vitro and performed in vivo studies in 6 subjects. Using Etafilcon A SCLs and 4M guanidine-HCl for protein removal, we sampled an average of 60 ± 31 µg of protein per eye. We also performed objective and subjective assessments of all sampling methods. Signs of irritation post-sampling were observed with SS but not with MCT and SCLs. Proteomic analysis by mass spectrometry (MS) revealed that all sampling methods resulted in the detection of abundant tear proteins. However, smaller subsets of unique and shared proteins were identified, particularly for SS and MCT. Additionally, there was no significant intrasubject variation between MCT and SCL sampling.

conclusionsThese experiments demonstrate that SCLs are an accessible tear-sampling method with the potential to surpass current methods in sampling basal tears.

Indexed as

Basal tearsEtafilcon AMass spectrometryProteomicsSoft contact lensesTear sampling

Identifiers

PMID38481131
PMCPMC10936081

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.