Evidence map›Paper›PMID 38478333›Full record

ReviewMethods in molecular biology (Clifton, N.J.)2024

Immunohistochemistry for Cancer Stem Cell Detection: Principles and Methods.

Giuseppa Zannini, Renato Franco, Federica Zito Marino

Abstract readReview
PubMed Publisher
In one paragraph

Review in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
2.3field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Giuseppa ZanniniPathology Unit, Dipartimento di Salute Mentale, Fisica e Medicina Preventiva, Università degli Studi della Campania 'Luigi Vanvitelli', Naples, Italy.
Renato FrancoPathology Unit, Dipartimento di Salute Mentale, Fisica e Medicina Preventiva, Università degli Studi della Campania 'Luigi Vanvitelli', Naples, Italy. renato.franco@unicampania.it.
Federica Zito MarinoPathology Unit, Dipartimento di Salute Mentale, Fisica e Medicina Preventiva, Università degli Studi della Campania 'Luigi Vanvitelli', Naples, Italy.
University of Campania "Luigi Vanvitelli" · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cancer stem cells (CSCs) are rare immortal cells within tumors with capabilities of self-renewal, differentiation, and tumorigenicity. CSCs play a pivotal role in the tumor development, progression, relapse, and resistance of anticancer therapy. The technique of choice to detect CSCs in formalin-fixed and paraffin-embedded (FFPE) samples is immunohistochemistry (IHC) since it is inexpensive and widespread in most laboratories. The main aims of this chapter are the description of the protocols and the automated immunohistochemical systems used for the identification of CSCs. Furthermore, a focus on the most common troubleshooting in CSC IHC is provided. Finally, an overview of the main markers of cancer stem cells in several cancer types will be provided.

Indexed as

NeoplasmsCell DifferentiationHumansImmunohistochemistryNeoplastic Stem CellsAntigen retrievalCancer stem cells (CSCs)Detection methodsFixationImmunohistochemistryStandardizationTroubleshooting

Identifiers

PMID38478333
OpenAlexW4392776161

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.