Stress-Based Screening for Compounds That Inhibit β-Barrel Outer Membrane Protein Assembly in Gram-Negative Bacteria.

Laurence Cleenewerk, Joen Luirink, Peter van Ulsen

Open access · greenAbstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

3.2field-weighted citation impact, top 11% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Laurence CleenewerkSection Molecular Microbiology, A-life Department and Amsterdam Institute of Molecular and Life Sciences, Vrije Universiteit, Amsterdam, The Netherlands.ORCID 0000-0003-3538-0288

Joen LuirinkSection Molecular Microbiology, A-life Department and Amsterdam Institute of Molecular and Life Sciences, Vrije Universiteit, Amsterdam, The Netherlands.ORCID 0000-0001-8431-0804

Peter van UlsenSection Molecular Microbiology, A-life Department and Amsterdam Institute of Molecular and Life Sciences, Vrije Universiteit, Amsterdam, The Netherlands. j.p.van.ulsen@vu.nl.ORCID 0000-0002-2164-1611

Vrije Universiteit Amsterdam · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Biogenesis of the outer membrane (OM) of Gram-negative bacteria involves two processes essential for growth, that is, the insertion of β-barrel outer membrane proteins (OMPs) by the Bam complex and the assembly of the LPS-containing outer leaflet of the OM by the LptD/E complex from the Lpt pathway. These processes have only recently gained attention as targets for antimicrobial drugs. Our laboratory has developed a simple screening tool to identify compounds that target processes that disrupt the biogenesis of the cell envelope, among which the activity of the Bam complex. The tool is based on the observation that such a disruption triggers cell envelope stress response systems, such as the σ

Indexed as

Escherichia coli ProteinsBacterial Outer Membrane ProteinsCell MembraneEscherichia coliGram-Negative BacteriaBacterial Outer Membrane ProteinsEscherichia coli ProteinsAntimicrobial drugsCell envelopeGram-negativeMembrane integrityOuter membranePhenotypic screeningStress responsesβ-barrel membrane proteins

Identifiers

PMID38478289

DOI10.1038/s41586-019-1791-1

OpenAlexW4392750774

What OpenQuestion holds

Textmetadata

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.