Evidence map›Paper›PMID 38478281›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Tracking the Activity and Position of Mitochondrial β-Barrel Proteins.

Shuo Wang, Stephan Nussberger

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Shuo WangBiophysics Department, Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Stuttgart, Germany.
Stephan NussbergerBiophysics Department, Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Stuttgart, Germany. stephan.nussberger@bio.uni-stuttgart.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Total interference reflection fluorescence (TIRF) microscopy of lipid bilayers is an effective technique for studying the lateral movement and ion channel activity of single integral membrane proteins. Here we describe how to integrate the mitochondrial outer membrane preprotein translocase TOM-CC and its β-barrel protein-conducting channel Tom40 into supported lipid bilayers to identify possible relationships between movement and channel activity. We propose that our approach can be readily applied to membrane protein channels where transient tethering to either membrane-proximal or intramembrane structures is accompanied by a change in channel permeation.

Indexed as

Mitochondrial ProteinsSaccharomyces cerevisiae ProteinsIon ChannelsLipid BilayersMitochondriaMitochondrial Membrane Transport ProteinsIon ChannelsLipid BilayersMitochondrial Membrane Transport ProteinsMitochondrial ProteinsSaccharomyces cerevisiae ProteinsChannelsMitochondriaProtein translocationSingle moleculeTIRF microscopyTOMβ-barrel membrane protein

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.