Evidence map›Paper›PMID 38478273›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Examining Protein Translocation by β-Barrel Membrane Proteins Using Reconstituted Proteoliposomes.

Minh Sang Huynh, Jiaming Caitlyn Xu, Trevor F Moraes

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
3.2field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Minh Sang HuynhDepartment of Biochemistry, University of Toronto, Toronto, ON, Canada.
Jiaming Caitlyn XuDepartment of Biochemistry, University of Toronto, Toronto, ON, Canada.
Trevor F MoraesDepartment of Biochemistry, University of Toronto, Toronto, ON, Canada. trevor.moraes@utoronto.ca.
MaRS · CAUniversity of Toronto · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

β-barrel membrane proteins populate the outer membrane of Gram-negative bacteria, mitochondria, and chloroplasts, playing significant roles in multiple key cellular pathways. Characterizing the functions of these membrane proteins in vivo is often challenging due to the complex protein network in the periplasm of Gram-negative bacteria (or intermembrane space in mitochondria and chloroplasts) and the presence of other outer membrane proteins. In vitro reconstitution into lipid-bilayer-like environments such as nanodiscs or proteoliposomes provides an excellent method for examining the specific function and mechanism of these membrane proteins in an isolated system. Here, we describe the methodologies employed to investigate Slam, a 14-stranded β-barrel membrane protein also known as the type XI secretion system that is responsible for translocating proteins across the outer membrane of many bacterial species.

Indexed as

Bacterial Outer Membrane ProteinsProteolipidsGram-Negative BacteriaMitochondriaProtein TransportBacterial Outer Membrane ProteinsProteolipidsproteoliposomesDenatured protein translocationGram-negative bacteriaIn vitro translocationouter membrane proteinsProteoliposome reconstitutionSpheroplast-released translocationSurface lipoprotein translocationType XI Secretion Systemβ-barrel membrane protein

Identifiers

PMID38478273
OpenAlexW4392820966

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.